BIOLOGY
BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 26.4, Problem 1LO

Describe the differences between acute and persistent infections.

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You intend to insert patched dominant negative DNA into the left half of the neural tube of a chick.  1) Which side of the neural tube would you put the positive electrode to ensure that the DNA ends up on the left side?  2) What would be the internal (within the embryo) control for this experiment?  3) How can you be sure that the electroporation method itself is not impacting the embryo?  4) What would you do to ensure that the electroporation is working? How can you tell?
Describe a method to document the diffusion path and gradient of Sonic Hedgehog through the chicken embryo. If modifying the protein, what is one thing you have to consider in regards to maintaining the protein’s function?
The following table is from Kumar et. al. Highly Selective Dopamine D3 Receptor (DR) Antagonists and Partial Agonists Based on Eticlopride and the D3R Crystal Structure: New Leads for Opioid Dependence Treatment. J. Med Chem 2016.
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