How the properties of T7 RNAP make it useful in experiments with recombinant DNA. Introduction: RNA polymerase (RNAP) enzymes are DNA-dependent RNA polymerases. In all species, transcription initiates through binding of RNA polymerase enzyme at the promoter region (beginning of gene).

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Chapter 26, Problem 26CQ

Summary Introduction

To explain: How the properties of T7 RNAP make it useful in experiments with recombinant DNA.

Introduction: RNA polymerase (RNAP) enzymes are DNA-dependent RNA polymerases. In all species, transcription initiates through binding of RNA polymerase enzyme at the promoter region (beginning of gene).

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[s] mM V (M/s) Uninhibited 0.333 1.65 x 107 1.05 x 107 V (M/s) x 10' Inhibitor A V (M/s) x 107 Inhibitor B 0.794 x 107 0.40 1.86 x 107 1.21 x 107 0.893 x 107 0.50 2.13 x 107 1.43 x 107 1.02 x 107 0.666 2.49 x 107 1.74 x 107 1.19 x 107 1.0 2.99 x 107 2.22 x 107 1.43 x 107 2.0 3.72 x 107 3.08 x 107 1.79 x 107

For a Michaelis-Menten reaction, k₁-5 x 10'/M-s, k.-2 x 10%/s, and k₂-4 x 10²/s. a) Calculate the Ks and KM for this reaction. b) Does substrate binding achieve equilibrium or steady state?

Assume that an enzyme-catalyzed reaction follows the scheme shown: E+S SES →E + P k₁ = 1 x 109/M-s k-1=2.5 x 10%/s k₂ = 3.4 x 107/s What is the dissociation constant for the enzyme-substrate, K,? What is the Michaelis constant, Km, for this enzyme? What is the turnover number, Keat, for this enzyme? What is the catalytic efficiency for the enzyme? If the initial Et concentration is 0.25mM, what is Vmax?

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Chapter 26, Problem 26CQ

Summary Introduction

To explain: How the properties of T7 RNAP make it useful in experiments with recombinant DNA.

Introduction: RNA polymerase (RNAP) enzymes are DNA-dependent RNA polymerases. In all species, transcription initiates through binding of RNA polymerase enzyme at the promoter region (beginning of gene).

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Chapter 26, Problem 26CQ

Summary Introduction

To explain: How the properties of T7 RNAP make it useful in experiments with recombinant DNA.

Introduction: RNA polymerase (RNAP) enzymes are DNA-dependent RNA polymerases. In all species, transcription initiates through binding of RNA polymerase enzyme at the promoter region (beginning of gene).

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Answer 4

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Chapter 26, Problem 26CQ

Summary Introduction

To explain: How the properties of T7 RNAP make it useful in experiments with recombinant DNA.

Introduction: RNA polymerase (RNAP) enzymes are DNA-dependent RNA polymerases. In all species, transcription initiates through binding of RNA polymerase enzyme at the promoter region (beginning of gene).

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Answer 5

Chapter 26, Problem 26CQ

Summary Introduction

To explain: How the properties of T7 RNAP make it useful in experiments with recombinant DNA.

Introduction: RNA polymerase (RNAP) enzymes are DNA-dependent RNA polymerases. In all species, transcription initiates through binding of RNA polymerase enzyme at the promoter region (beginning of gene).

Answer 6

Chapter 26, Problem 26CQ

Summary Introduction

To explain: How the properties of T7 RNAP make it useful in experiments with recombinant DNA.

Introduction: RNA polymerase (RNAP) enzymes are DNA-dependent RNA polymerases. In all species, transcription initiates through binding of RNA polymerase enzyme at the promoter region (beginning of gene).

Answer 7

Chapter 26, Problem 26CQ

Summary Introduction

To explain: How the properties of T7 RNAP make it useful in experiments with recombinant DNA.

Introduction: RNA polymerase (RNAP) enzymes are DNA-dependent RNA polymerases. In all species, transcription initiates through binding of RNA polymerase enzyme at the promoter region (beginning of gene).

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Ch. 26 - Prob. 1E Ch. 26 - 2. The bacterial enzyme polynucleotide...Ch. 26 - Prob. 3E Ch. 26 - Prob. 4E Ch. 26 - Prob. 5E Ch. 26 - Prob. 6E Ch. 26 - Prob. 7E Ch. 26 - Prob. 8E Ch. 26 - Prob. 9E Ch. 26 - 10. Certain E. coli bacteriophages encode a...

How the properties of T7 RNAP make it useful in experiments with recombinant DNA. Introduction: RNA polymerase (RNAP) enzymes are DNA-dependent RNA polymerases. In all species, transcription initiates through binding of RNA polymerase enzyme at the promoter region (beginning of gene).

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To explain: How the properties of T7 RNAP make it useful in experiments with recombinant DNA.

Introduction: RNA polymerase (RNAP) enzymes are DNA-dependent RNA polymerases. In all species, transcription initiates through binding of RNA polymerase enzyme at the promoter region (beginning of gene).

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Chapter 26 Solutions

How the properties of T7 RNAP make it useful in experiments with recombinant DNA. Introduction: RNA polymerase (RNAP) enzymes are DNA-dependent RNA polymerases. In all species, transcription initiates through binding of RNA polymerase enzyme at the promoter region (beginning of gene).

Concept explainers

Videos

To explain: How the properties of T7 RNAP make it useful in experiments with recombinant DNA. Introduction: RNA polymerase (RNAP) enzymes are DNA-dependent RNA polymerases. In all species, transcription initiates through binding of RNA polymerase enzyme at the promoter region (beginning of gene).

Want to see the full answer?

Chapter 26 Solutions

To explain: How the properties of T7 RNAP make it useful in experiments with recombinant DNA.

Introduction: RNA polymerase (RNAP) enzymes are DNA-dependent RNA polymerases. In all species, transcription initiates through binding of RNA polymerase enzyme at the promoter region (beginning of gene).