The reactions underlying the observations that when 32 P-labeled DNA and unlabeled dNTPs are added, 32 PdNMPs are not released. Introduction : DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

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Answer 1

Question

Chapter 25, Problem 7P

Summary Introduction

To explain: The reactions underlying the observations that when ³²P-labeled DNA and unlabeled dNTPs are added, ³²PdNMPs are not released.

Introduction:

DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

Summary Introduction

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

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Answer 2

Question

Chapter 25, Problem 7P

Summary Introduction

To explain: The reactions underlying the observations that when ³²P-labeled DNA and unlabeled dNTPs are added, ³²PdNMPs are not released.

Introduction:

DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

Summary Introduction

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

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Answer 3

Question

Chapter 25, Problem 7P

Summary Introduction

To explain: The reactions underlying the observations that when ³²P-labeled DNA and unlabeled dNTPs are added, ³²PdNMPs are not released.

Introduction:

DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

Summary Introduction

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

Expert Solution & Answer

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Answer 4

Question

Chapter 25, Problem 7P

Summary Introduction

To explain: The reactions underlying the observations that when ³²P-labeled DNA and unlabeled dNTPs are added, ³²PdNMPs are not released.

Introduction:

DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

Summary Introduction

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

Expert Solution & Answer

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Answer 5

Chapter 25, Problem 7P

Summary Introduction

To explain: The reactions underlying the observations that when ³²P-labeled DNA and unlabeled dNTPs are added, ³²PdNMPs are not released.

Introduction:

DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

Summary Introduction

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

Answer 6

Chapter 25, Problem 7P

Summary Introduction

To explain: The reactions underlying the observations that when ³²P-labeled DNA and unlabeled dNTPs are added, ³²PdNMPs are not released.

Introduction:

DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

Answer 7

Chapter 25, Problem 7P

Summary Introduction

To explain: The reactions underlying the observations that when ³²P-labeled DNA and unlabeled dNTPs are added, ³²PdNMPs are not released.

Introduction:

DNA polymerase enzyme synthesizes the addition of nucleotides leading to the formation of phosphodiester bonds between the deoxyribose sugar group and nucleotides.

Answer 8

Summary Introduction

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

Answer 9

Summary Introduction

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

Answer 10

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Answer 11

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Answer 13

Ch. 25 - Prob. 1P Ch. 25 - Prob. 2P Ch. 25 - Prob. 3P Ch. 25 - Prob. 4P Ch. 25 - Prob. 5P Ch. 25 - Prob. 6P Ch. 25 - Prob. 7P Ch. 25 - Prob. 8P Ch. 25 - Prob. 9P Ch. 25 - Prob. 10P

Concept explainers

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

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Chapter 25 Solutions

Concept explainers

To determine: What is expected if pyrophosphate is added instead of dNTPs . Introduction: dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.

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Chapter 25 Solutions

To determine: What is expected if pyrophosphate is added instead of dNTPs .

Introduction:

dNTPs are deoxynucleotides which consist of three phosphate groups attached at the 5'OH(hydroxyl) end of deoxyribose sugar.