PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 2818440045677
Author: WILLEY
Publisher: MCG
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Textbook Question
Chapter 25, Problem 4AL
Analysis of sclerotia and conidia formation in the filamentous ascomycete Aspergillus flavus suggests that formation of these structures is cell density dependent. Specifically, high cell density cultures yield conidia, whereas low cell density triggers sclerotia formation. Review the structure and function of conidia and sclerotia, and formulate a hypothesis to explain why their development would be regulated in a density-dependent manner. List several specific testable predictions based on your hypothesis.
Read the original paper: Horowitz-Brown, S., et al. 2008. Morphological transitions governed by density dependence and lipooxygenase activity in Aspergillus flavus. Appl. Environ. Microbiol. 74:5674.
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a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Chapter 25 Solutions
PRESCOTT'S MICROBIOLOGY
Ch. 25.1 - Prob. 1CCCh. 25.1 - What is the distribution of these microbes?Ch. 25.1 - Prob. 3CCCh. 25.1 - Prob. 4CCCh. 25.1 - Prob. 5CCCh. 25.2 - Prob. 1MICh. 25.4 - Prob. 1CCCh. 25.4 - What is the difference between hyphae called...Ch. 25.4 - How do different mating types of Rhizopus...Ch. 25.4 - Prob. 4CC
Ch. 25.4 - Prob. 5CCCh. 25.5 - What determines when a yeast cell can no longer...Ch. 25.5 - In what ways is the life cycle of filamentous...Ch. 25.5 - Why does nutrient deprivation trigger sexual...Ch. 25.5 - Prob. 2CCCh. 25.5 - Where are you most likely find ascomycetes that...Ch. 25.6 - Prob. 1MICh. 25.7 - Prob. 1CCCh. 25.7 - Prob. 2CCCh. 25.7 - Prob. 3CCCh. 25.7 - Why do you think members of Microsporidia are...Ch. 25 - Prob. 1RCCh. 25 - Prob. 2RCCh. 25 - Prob. 3RCCh. 25 - Prob. 4RCCh. 25 - Prob. 5RCCh. 25 - Prob. 1ALCh. 25 - Prob. 2ALCh. 25 - Prob. 3ALCh. 25 - Analysis of sclerotia and conidia formation in the...Ch. 25 - Prob. 5AL
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