Biology
Biology
11th Edition
ISBN: 9781259188138
Author: Peter H Raven, George B Johnson Professor, Kenneth A. Mason Dr. Ph.D., Jonathan Losos Dr., Susan Singer
Publisher: McGraw-Hill Education
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Chapter 25, Problem 1S

The paired-like homeodomain transcription factor 1 (pitxl) is expressed in the hindlimbs of developing mouse embryos, and its homologue is expressed in the pelvic region of the nine-spine stickleback fish (Pungitius pungitius). You are beginning a research project on pitxl and your supervisor is convinced that pitxl has been co-opted to make armor around the pelvic region of this species of stickleback. You begin by isolating RNA from a freshwater Pungitius pungitius that has very reduced armor. You convert the RNA to cDNA and amplify the pitxl cDNA using PCR. You have your pitxl PCR product sequenced and find it has exactly the same sequence as the marine Pungitius pungitius with armor. In light of this evidence, analyze your supervisor's hypothesis.

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