Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
bartleby

Videos

Textbook Question
Book Icon
Chapter 25, Problem 1Q

Is the disk-diffusion technique measuring bacteriostatic or bactericidal activity? Briefly explain.

Expert Solution & Answer
Check Mark
Summary Introduction

To write:

The nature of the activity of the disc-diffusion technique.

Introduction:

The disc-diffusion method is also called Kirby-Bauer test. It is a technique that tests the effectiveness of the antibiotics on a specific microorganism. An agar plate is spread with bacteria and then, paper discs of antibiotics are added to it. The bacteria are allowed to grow on the agar media and inhibition zones are observed.

Explanation of Solution

Antibiotics eliminate the bacteria completely. They are categorized as bactericidal, that is potent and bacteriostatic, which hampers growth. Bactericidal antibiotics kill the bacteria directly while the bacteriostatic antibiotics inhibit the bacteria from growing. The disc-diffusion technique works both by inhibiting the growth as well as by killing the bacteria.

The zone of inhibition is formed around the antibiotic discs. Bigger is the area, maximum will be the activity of the antibiotic. Similarly, lesser is the area, the minimum will be the activity of the antibiotic. Around the antibiotic discs, bacteria are not grown in any case nor the bacteria have been killed. They kill the bacteria as well as inhibit the growth also. They act both ways of the action.

Conclusion

Thus, disc-diffusion technique measured both the ways of bactericidal as well as bacteriostatic activity as they inhibit bacterial growth and killed the bacteria as well.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!
Students have asked these similar questions
Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…
Question #5: Assume that two genes are identified that confer gametophytic facultative apomixis in soybean. The genes show independent assortment. Recessive alleles at both loci are required for the facultative apomixis. Facultative apomixis is triggered when the temperature at pollination is above 20 degrees C. At temperatures below 20 degrees C, all reproduction is sexual, independent of genotype. A facultative apomict male, capable of producing viable pollen, was crossed with a sexually reproducing female. Assuming the parents are completely inbred, what are the predicted phenotypic ratios (apomict: non-apomict) for the F1, F2, and DH (F1-derived) generations at each of the following temperatures*: a) 15°C? b) 25°C? *for full credit, show crosses and genotypes where appropriate. Remember to position the female first (left side) in the cross. Type your answer here:
a. What percentage of a drug is eliminated after 4 half-lives? Please round to the nearest percent.  b. What will happen to elimination of the drug in the previous question if the system is saturated? explain and show any math involved
Knowledge Booster
Background pattern image
Biology
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Basic Clinical Laboratory Techniques 6E
Biology
ISBN:9781133893943
Author:ESTRIDGE
Publisher:Cengage
Text book image
Curren'S Math For Meds: Dosages & Sol
Nursing
ISBN:9781305143531
Author:CURREN
Publisher:Cengage
Text book image
Principles Of Radiographic Imaging: An Art And A ...
Health & Nutrition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Cengage Learning
Text book image
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning
Text book image
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Text book image
Aquaculture Science
Biology
ISBN:9781133558347
Author:Parker
Publisher:Cengage
Biochemical Tests-Part 1; Author: Southern Stacker;https://www.youtube.com/watch?v=a-i9vANfQWQ;License: Standard Youtube License