Genetics: Analysis and Principles
Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 24.1, Problem 1COMQ

A DNA microarray is a slide that is dotted with

a. mRNAs from a sample of cells.

b. fluorescently labeled cDNA.

c. known sequences of DNA.

d. known cellular proteins.

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Place the steps of sanger sequencing in order.A.  A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B.  ddNTPs bind and stop chain extension.C.  DNA fragments are separated by size through a capillary tube. D.  DNA polymerase copies the target region of template DNA.E.  The final nucleotide of each fragment is labeled with a fluorescent tag.
A contig is a. a set of molecular markers used in gene mapping. b. a set of overlapping fragments that form a continuous stretch of DNA. c. a set of fragments generated by a restriction enzyme. d. a small DNA fragment used in sequencing.
Place the following stages of a physical mapping study in theirmost logical order:A. Clone large fragments of DNA to make a BAC library.B. Determine the DNA sequence of subclones from a cosmidlibrary.C. Subclone BAC fragments to make a cosmid library.D. Subclone cosmid fragments for DNA sequencing.
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