Chapter 24, Problem 18E

Summary Introduction

To explain: The results obtained from restriction digestion and electrophoresis of mouse genomic DNA.

Concept introduction: Specific DNA molecules are cleaved using restriction digestion followed by electrophoresis. Restriction enzyme cuts the DNA molecule at different positions to yield fragments of DNA of varying lengths. Agarose gel electrophoresis is one of the methods used to separate and analyze DNA molecules. The DNA molecules migrate to different distances based on their size and charge and precipitate as bands over the agarose gel. The mobility of the DNA on the gel depends on the conformation of the DNA.