Microbiology: An Introduction, Books a la Carte Plus Mastering Microbiology with Pearson eText -- Access Card Package (13th Edition)
Microbiology: An Introduction, Books a la Carte Plus Mastering Microbiology with Pearson eText -- Access Card Package (13th Edition)
13th Edition
ISBN: 9780134729336
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 24, Problem 10R
Summary Introduction

To review:

Bordetella pertussis is the aerobic, gram-negative bacteria which produces cytotoxin kills ciliated cells of the trachea.

Introduction:

Whooping cough is an upper respiratory system infection which is also called pertussis, leads to uncontrollable cough and intake of high pitch air so called whooping cough. It is caused by Bordetella pertussis. Its infections resembles common cold, vomiting and pneumonia.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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