SaplingPlus for Lehninger Principles of Biochemistry (Six-Month Access)
SaplingPlus for Lehninger Principles of Biochemistry (Six-Month Access)
7th Edition
ISBN: 9781319108236
Author: David L. Nelson, Michael M. Cox
Publisher: W. H. Freeman
Question
Book Icon
Chapter 23, Problem 9P

(a)

Summary Introduction

To determine: The importance of relatively rapid inactivation of circulating hormone.

Introduction:

Hormone is an organic substance that is secreted by plants and animals. Hormones regulate many physiological activities. Hormone is made up of protein. It is transported in our body through circulatory system. In blood, regulation of hormone becomes half when radioactively labeled insulin is introduced into an animal. Half of the labeled hormone disappears from the blood.

(b)

Summary Introduction

To determine: The way by which the level of circulating hormone remains constant under normal conditions with respect to rapid inactivation.

Introduction:

Hormone is an organic substance that is secreted by plants and animals. Hormones regulate many physiological activities. Hormone is made up of protein. It is transported in our body through circulatory system. In blood, regulation of hormone becomes half when radioactively labeled insulin is introduced into an animal. Half of the labeled hormone disappears from the blood.

(c)

Summary Introduction

To determine: The changes that an organism makes in the level of circulating hormone.

Introduction:

Hormone is an organic substance that is secreted by plants and animals. Hormones regulate many physiological activities. Hormone is made up of protein. It is transported in our body through circulatory system. In blood, regulation of hormone becomes half when radioactively labeled insulin is introduced into an animal. Half of the labeled hormone disappears from the blood.

Blurred answer
Students have asked these similar questions
Draw a diagram to demonstrate 3' to 5' exonuclease activity?
1. Write the piece of mRNA that would code for the amino acid sequence NH3 - met - tyr - cys- his- CO2.  Label the 5' and 3' ends. (diagram attached)  2.
You were tasked with purifying an untagged transcription factor (molecular weight 65,000Da, isoelectric point unknown) from a contaminant Protein A (molecular weight 50,000 Da, isoelectric point 5.0). You were also instructed to use the protein for crystallization studies after purification.i) In the initial purification step, which type of chromatography should you attempt? Explain your choice and specify the requirements of the buffer solution you should use. ii) Analyzing the proteins recovered from step i) using SDS-PAGE, you still observed a faint band of Protein A in addition to the transcription factor band. Given the limited time available for further purification, you must choose ONE appropriate chromatography method to maximize the chances of separating the two proteins as well as your crystallization studies. Provide a detailed explanation of your selection and the techniques/strategies you would employ to achieve this.
Knowledge Booster
Background pattern image
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Biochemistry
Biochemistry
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:W. H. Freeman
Text book image
Lehninger Principles of Biochemistry
Biochemistry
ISBN:9781464126116
Author:David L. Nelson, Michael M. Cox
Publisher:W. H. Freeman
Text book image
Fundamentals of Biochemistry: Life at the Molecul...
Biochemistry
ISBN:9781118918401
Author:Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:WILEY
Text book image
Biochemistry
Biochemistry
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Cengage Learning
Text book image
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Text book image
Fundamentals of General, Organic, and Biological ...
Biochemistry
ISBN:9780134015187
Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:PEARSON