Chapter 23, Problem 23.20P

(a)

Interpretation Introduction

Interpretation:

The linear velocity has to be calculated for the volume flow rate of $\text{1}\text{.13}\text{mL/min}\text{.}$

(b)

Interpretation Introduction

Interpretation:

The time taken by solvent to pass through the column has to be calculated.

Concept introduction:

Retention factor:

The retention factor $\left(\text{k}\right)$ is given by

$\begin{array}{l}\text{k}\text{=}\frac{{\text{t}}_{\text{r}}\text{-}{\text{t}}_{\text{m}}}{{\text{t}}_{\text{m}}}\\ \text{Where,}\\ \text{k}\text{-}\text{retention}\text{factor}\\ {\text{t}}_{\text{r}}\text{-}\text{time}\text{required}\text{to}\text{elute}\text{the}\text{peak}\\ {\text{t}}_{\text{m}}\text{-}\text{time}\text{required}\text{for}\text{mobile}\text{phase}\text{to}\text{pass}\text{through}\text{the}\text{column}\text{.}\end{array}$

(c)

Interpretation Introduction

Interpretation:

The retention time for a solute with retention factor of 10.0 has to be calculated.

Concept introduction:

Retention factor:

The retention factor $\left(\text{k}\right)$ is given by

$\begin{array}{l}\text{k}\text{=}\frac{{\text{t}}_{\text{r}}\text{-}{\text{t}}_{\text{m}}}{{\text{t}}_{\text{m}}}\\ \text{Where,}\\ \text{k}\text{-}\text{retention}\text{factor}\\ {\text{t}}_{\text{r}}\text{-}\text{time}\text{required}\text{to}\text{elute}\text{the}\text{peak}\\ {\text{t}}_{\text{m}}\text{-}\text{time}\text{required}\text{for}\text{mobile}\text{phase}\text{to}\text{pass}\text{through}\text{the}\text{column}\text{.}\end{array}$