Biology
12th Edition
ISBN: 9781260494570
Author: Raven, Peter
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Question
Chapter 2.2, Problem 2LO
Summary Introduction
To list: The important elements present in the living systems.
Introduction: An atom can be defined as the smallest componentunit of matter that comprises a chemical element. A particle that is made from the chemical combination of two atoms or more is called a molecule. When atoms of distinct elements bind together, they form molecules known as compounds.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 2 Solutions
Biology
Ch. 2.1 - Define an element based on its composition.Ch. 2.1 - Describe the relationship between atomic structure...Ch. 2.1 - Explain where electrons are found in an atom.Ch. 2.2 - Relate atomic structure to the periodic table of...Ch. 2.2 - Prob. 2LOCh. 2.3 - Prob. 1LOCh. 2.3 - Prob. 2LOCh. 2.3 - Prob. 3LOCh. 2.4 - Relate how the structure of water leads to...Ch. 2.4 - Prob. 2LO
Ch. 2.5 - Prob. 1LOCh. 2.5 - Prob. 2LOCh. 2.5 - Prob. 3LOCh. 2.6 - Prob. 1LOCh. 2.6 - Prob. 2LOCh. 2 - Data analysis If we call each step on the x-axis...Ch. 2 - The property that distinguishes an atom of one...Ch. 2 - If an atom has one valence electronthat is. a...Ch. 2 - An atom with a net positive charge must have more...Ch. 2 - The isotopes carbon-12 and carbon-14 differ in a....Ch. 2 - Which of the following is NOT a property of the...Ch. 2 - Ionic bonds arise from a. shared valence...Ch. 2 - A solution with a high concentration of hydrogen...Ch. 2 - Using the periodic table on page 22, which of the...Ch. 2 - Refer to the element pictured. How many covalent...Ch. 2 - Prob. 3ACh. 2 - Prob. 4ACh. 2 - If you shake a bottle of oil and vinegar then let...Ch. 2 - The decay of radioactive isotopes involves changes...Ch. 2 - Prob. 1SCh. 2 - A popular theme in science fiction literature has...Ch. 2 - Prob. 3S
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