MICRO. FUND. CONNECT CODE W/VIRTUAL LAB
MICRO. FUND. CONNECT CODE W/VIRTUAL LAB
3rd Edition
ISBN: 9781266313806
Author: Cowan
Publisher: INTER MCG
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Chapter 22, Problem 16Q
Summary Introduction

Introduction:

Infection is caused in an individual body by disease-causing agents. In infection, the disease-causing agent multiplies and release the toxin inside the host body. The host cells will develop a certain reaction to inhibit the action of these disease-causing agents by generating an immune response against it. Infection can be caused by bacteria, fungi or protozoa. Infection ranges from inflammation in one person to an epidemic.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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Mechanisms of Pathogenicity: Microbiology; Author: Dr. Frank O'Neill GrowGrayMatter;https://www.youtube.com/watch?v=SDyl0JNCeho;License: CC-BY