Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 21.8, Problem 1CR
Summary Introduction
Microorganisms are responsible for the breakdown of organic matter and some also contribute in releasing greenhouse gases. Various activities of microbes lead to global climate change and also minimize emission of various gases. It has been observed that microbial processes play a central role in regulating global fluxes of greenhouse gases and rapidly respond to climate changes.
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Part A: The Effect of Temperature on Enzyme Activity
Graph the volume of oxygen produced against the temperature of the solution.
How is the oxygen production in 30 seconds related to the rate of the reaction?
At what temperature is the rate of reaction the highest? Lowest? Explain.
Why might the enzyme activity decrease at very high temperatures?
Why might a high fever be dangerous to humans?
What is the optimal temperature for enzymes in the human body?
Part B: The Effect of pH on Enzyme Activity
Graph the volume of oxygen produced against the pH of the solution.
At what pH is the rate of reaction the highest? Lowest? Explain.
Why does changing the pH affect the enzyme activity?
Research the enzyme catalase. What is its function in the human body?
What is the optimal pH for the following enzymes found in the human body? Explain. (catalase, lipase (in your stomach),…
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Discussion Questions:
Part One
Why were the plants kept in the dark prior to the experiment? Why is this important?
Why is it important to boil the leaf?
Explain why it was necessary to use boiling alcohol?
What is the purpose of the iodine?
Part Two
What was the purpose of keeping the leaf in the dark and then covering it with a cardboard cut-out?
What conclusions can you draw from this part of the lab?
Part Three
7. In this experiment what was the purpose of adding the soda lime?
8. Why was a sealed bag placed around each plant?
9. What happened in the control plants?
10. What was the result on photosynthesis?
Part Four
11. Why was a variegated leaf used in this experiment?
!2. What conclusions can you draw about starch production in a variegated leaf?
How did the color differences between the two bacterial species you used in this experiment help you determine if the streak plate method you performed was successful?
Chapter 21 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 21.1 - How is new organic matter made in nature?Ch. 21.1 - In what ways are oxygenic photosynthesis and...Ch. 21.1 - What is a methane hydrate?Ch. 21.1 - The oxygen and carbon cycles are interconnected...Ch. 21.2 - Why does Syntrophomonas need a partner organism in...Ch. 21.2 - Prob. 2MQCh. 21.2 - What is the final product of acetogenesis?Ch. 21.2 - Prob. 1CRCh. 21.3 - What is nitrogen fixation and why is it important...Ch. 21.3 - Prob. 2MQ
Ch. 21.3 - How does the compound nitrapyrin benefit both...Ch. 21.3 - The principal form of nitrogen on Earth is N2,...Ch. 21.4 - Is H2S a substrate or a product of the...Ch. 21.4 - Prob. 2MQCh. 21.4 - Prob. 3MQCh. 21.4 - Bacteria play major roles in both the oxidative...Ch. 21.5 - In what oxidation state is Fe in Fe(OH)3? In FeS?...Ch. 21.5 - Prob. 2MQCh. 21.5 - Why is excreted organic matter important to many...Ch. 21.5 - Prob. 1CRCh. 21.6 - How does the formation of CaCO3 skeletons by...Ch. 21.6 - Prob. 2MQCh. 21.6 - Prob. 1CRCh. 21.7 - What forms of mercury are most toxic to organisms?Ch. 21.7 - Prob. 2MQCh. 21.7 - Prob. 3MQCh. 21.7 - Prob. 1CRCh. 21.8 - What is the greenhouse effect and what causes it?Ch. 21.8 - What is the fate of most nitrogen used in...Ch. 21.8 - Why are the OMZs expanding and what are the likely...Ch. 21.8 - Prob. 1CRCh. 21 - Compare and contrast the carbon, sulfur, and...Ch. 21 - 14C-labeled cellulose is added to a vial...Ch. 21 - Prob. 3AQ
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