BROOKER BIOLOGY
5th Edition
ISBN: 9781307656152
Author: BROOKER
Publisher: MCG/CREATE
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Textbook Question
Chapter 21, Problem 2TY
DNA ligase is needed in a cloning experiment
- a. to promote hydrogen bonding between sticky ends.
- b. to covalently link the backbone of DNA strands.
- c. to digest the chromosomal DNA into small pieces.
- d. to do only a and b.
- e. to do a, b, and c.
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Place the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide.
B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube.
D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.
Which of the following is NOT true of DNA polymerase?
a. DNA polymerase builds the new strand from 5' to 3'.
b. DNA polymerase can start new DNA strands independently.
c. DNA polymerase uses a "guess and check" method to add nucleotides.
d. DNA polymerase uses dATP as a nucleotide.
e. DNA polymerase reads the template from 3' to 5'.
Which of the following is FALSE about current Sanger dideoxy DNA sequencing procedures?
a.
Chain termination occurs during synthesis of a new DNA strand.
b.
Many steps can be automated.
c.
No DNA is synthesized in the procedure.
d.
Fluorescent molecules can be used to detect the DNA.
Chapter 21 Solutions
BROOKER BIOLOGY
Ch. 21.1 - Prob. 1CSCh. 21.1 - Prob. 1CCCh. 21.1 - Prob. 2CCCh. 21.1 - Prob. 3CCCh. 21.2 - Prob. 1CCCh. 21.2 - Prob. 2CCCh. 21.2 - Prob. 3CCCh. 21.3 - Prob. 1EQCh. 21.3 - Prob. 2EQCh. 21.3 - Prob. 3EQ
Ch. 21.4 - Prob. 1CCCh. 21.4 - Prob. 1CSCh. 21.5 - Prob. 1CSCh. 21.5 - Repetitive Sequences and Transposable Elements...Ch. 21 - Prob. 1TYCh. 21 - DNA ligase is needed in a cloning experiment a. to...Ch. 21 - Prob. 3TYCh. 21 - Why is Taq polymerase used in PCR rather than...Ch. 21 - Lets suppose you want to clone a gene that has...Ch. 21 - In the CRISPR-Cas technology for editing genes,...Ch. 21 - Prob. 7TYCh. 21 - The enzyme that helps short segments of DNA move...Ch. 21 - Prob. 9TYCh. 21 - Which of the following was not a goal of the Human...Ch. 21 - Prob. 1CQCh. 21 - Briefly describe whether or not each of the...Ch. 21 - Prob. 3CQCh. 21 - Identify and discuss three important advances that...Ch. 21 - Prob. 2COQ
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Which of the following statements about DNA probes are false? a. Probes can be labeled with a fluorescent dye to facilitate detection. b. Probes can be labeled with radioactive phosphorus to facilitate detection. c. Probes are typically composed of double stranded pieces of DNA. d. Probes are typically composed of single stranded pieces of DNA.arrow_forwardA DNA microarray is a slide that is dotted witha. mRNAs from a sample of cells.b. fluorescently labeled cDNA.c. known sequences of DNA.d. known cellular proteins.arrow_forwardPut the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forward
- which of the following do researchers not need to use during vector cloning? a. a plasmid containing selectable marker genes such as beta galactosidase or ampicillin resistance genes. b. restriction enzymes. c. DNA polymerase d. a growth medium with carefully selected ingredients that take advantage of selectable markers. e. none of the above.arrow_forwardA genetic map provides a. the sequence of the DNA in a genome. b. the relative position of genes on chromosomes. c. the location of sites of restriction enzyme cleavage in a known sequence of DNA. d. the banding pattern of a chromosome.arrow_forwardThe function of DNA ligase is to: a. Catalyze formation of phosphodiester bonds between adjacent nucleotides b. Catalyze formation of hydrogen bonds between adjacent nucleotides c. Keep single strands of DNA apart during replication d. Facilitate base pairing between single stranded molecules in DNA e. Both a. and d. are correctarrow_forward
- What is a restriction endonuclease? Select one: a. It is an enzyme that cleaves at a specific nucleotide sequence. b. It restricts the movement of the DNA outside the nucleus. c. It proofreads the DNA for accidental damages and corrects any errors. d. It is an enzyme that separates the DNA double helix.arrow_forwardOne common feature of all DNA polymerases is that they a. synthesize DNA in the 3′-to-5′ direction. b. synthesize DNA in the 5′-to-3′ direction. c. synthesize DNA in both directions by switching strands. d. do not require a primer.arrow_forwardIn an EMSA, the binding of a protein to DNAa. prevents the DNA from being digested with a restrictionenzyme.b. causes the DNA to migrate more slowly through a gel.c. causes the DNA to migrate more quickly through a gel.d. inhibits the expression of any genes within the DNA.arrow_forward
- Certain restriction endonucleases produce cohesive (sticky) ends. This means that they: a. stick tightly to the ends of the DNA they have cut. b. cut both DNA strands at the same base pair. c. make a staggered double-strand cut, leaving ends with a few nucleotides of single-stranded DNA protruding. d. cut in regions of high GC content, leaving ends that can form more hydrogen bonds than ends of high AT content. e. cut in regions of high AT content, leaving ends that can form more hydrogen bonds than ends of high GC content.arrow_forwardIn nucleotide excision repair in E. coli, the function of the UvrA/UvrB complex is toa. detect DNA damage.b. make cuts on both sides of the damage.c. remove the damaged piece of DNA.d. replace the damaged DNA with undamaged DNA.arrow_forwardWhich of the following is true about DNA manipulation? Select the best answer. A. all of these B. DNA has never been transferred between different organisms. C. Transferring DNA from one organism into another always kills the host organism. D. Whole genomes have been manipulated by humans for centuries; now individual genes ca be inserted into different organisms using a variety of techniques. E. DNA can't be manipulated.arrow_forward
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