Loose Leaf For Anatomy & Physiology: An Integrative Approach
3rd Edition
ISBN: 9781260162493
Author: McKinley Dr., Michael; O'Loughlin, Valerie; Bidle, Theresa
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 21, Problem 2DYB
_____ 2. Lymph is drained into the thoracic duct from which of the following body regions?
- a. right lower limb
- b. right upper limb
- c. right side of the head
- d. right side of the thorax
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 21 Solutions
Loose Leaf For Anatomy & Physiology: An Integrative Approach
Ch. 21.1 - Prob. 1LOCh. 21.1 - Prob. 2LOCh. 21.1 - Prob. 3LOCh. 21.1 - What substances typically are absorbed from the...Ch. 21.1 - Prob. 2WDLCh. 21.1 - Prob. 4LOCh. 21.1 - Prob. 5LOCh. 21.1 - Prob. 6LOCh. 21.1 - Prob. 1WDTCh. 21.1 - Prob. 3WDL
Ch. 21.1 - Which major body regions drain lymph to the right...Ch. 21.2 - Prob. 7LOCh. 21.2 - How are primary lymphatic structures and secondary...Ch. 21.3 - Prob. 8LOCh. 21.3 - Prob. 9LOCh. 21.3 - Why is red bone marrow considered a primary...Ch. 21.3 - Prob. 10LOCh. 21.3 - Prob. 7WDLCh. 21.4 - Prob. 11LOCh. 21.4 - Prob. 12LOCh. 21.4 - Prob. 8WDLCh. 21.4 - Prob. 13LOCh. 21.4 - Prob. 14LOCh. 21.4 - Prob. 15LOCh. 21.4 - Prob. 2WDTCh. 21.4 - What are the general functions of the spleen?...Ch. 21.4 - Prob. 10WDLCh. 21.4 - Prob. 16LOCh. 21.4 - Prob. 11WDLCh. 21.4 - Prob. 17LOCh. 21.4 - LEARNING OBJECTIVE
18. Compare the locations of...Ch. 21.4 - What is the function of MALT in the mucosal...Ch. 21 - Prob. 1DYBCh. 21 - _____ 2. Lymph is drained into the thoracic duct...Ch. 21 - _____ 3. The spleen is a secondary lymphatic...Ch. 21 - Prob. 4DYBCh. 21 - _____ 5. Which type of lymph vessel consists...Ch. 21 - _____ 6. Which statement is accurate about lymph...Ch. 21 - In an early Streptococcus infection of the throat,...Ch. 21 - _____ 8. The lymphatic trunk that drains lymph...Ch. 21 - _____ 9. Aged erythrocytes are removed from...Ch. 21 - _____ 10. Interstitial fluid that is absorbed into...Ch. 21 - Prob. 11DYBCh. 21 - Prob. 12DYBCh. 21 - Prob. 13DYBCh. 21 - Which body regions have their lymph drained to the...Ch. 21 - Describe how the thymuss anatomy changes as we...Ch. 21 - Prob. 16DYBCh. 21 - Compare and contrast the red and white pulp of the...Ch. 21 - Describe the specific locations of the tonsils.Ch. 21 - Describe the location and function of diffuse...Ch. 21 - Prob. 20DYBCh. 21 - Prob. 1CALCh. 21 - A child born without his thymus would not have...Ch. 21 - Prob. 3CALCh. 21 - Prob. 4CALCh. 21 - Prob. 5CALCh. 21 - Prob. 1CSLCh. 21 - Jordan has an enlarged lymph node along the side...Ch. 21 - Prob. 3CSL
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