BIOCHEMISTRY II >CUSTOM<
BIOCHEMISTRY II >CUSTOM<
17th Edition
ISBN: 9781337449014
Author: GARRETT
Publisher: CENGAGE C
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Chapter 21, Problem 18P

The Overall Free Energy Change for Photosynthetic NADP+ Reduction

What is the overall free energy change ( Δ G ) for noncyclic photosynthetic electron transport?

4 (700-nm photons) + 4 (680-nm photons) + 2 H2O + 2 NADP+ O2 + 2 NADPH + 2H+

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Draw a diagram to demonstrate 3' to 5' exonuclease activity?
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You were tasked with purifying an untagged transcription factor (molecular weight 65,000Da, isoelectric point unknown) from a contaminant Protein A (molecular weight 50,000 Da, isoelectric point 5.0). You were also instructed to use the protein for crystallization studies after purification.i) In the initial purification step, which type of chromatography should you attempt? Explain your choice and specify the requirements of the buffer solution you should use. ii) Analyzing the proteins recovered from step i) using SDS-PAGE, you still observed a faint band of Protein A in addition to the transcription factor band. Given the limited time available for further purification, you must choose ONE appropriate chromatography method to maximize the chances of separating the two proteins as well as your crystallization studies. Provide a detailed explanation of your selection and the techniques/strategies you would employ to achieve this.
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