EBK HUMAN BIOLOGY
11th Edition
ISBN: 9780100545939
Author: MCMILLAN
Publisher: YUZU
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Chapter 21, Problem 10SQ
Summary Introduction
Introduction: Recombinant DNA is synthesized as a part of
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Okazaki fragments are ________.
A.
short RNA primers needed for initiation of polymerization
B.
fragments of DNA polymerase I that lack 5' → 3' exonuclease activity
C.
short stretches of DNA formed on the lagging strand
D.
the smallest subunits of DNA polymerase III
The initial mechanism for repairing nucleotide errors in DNA is ________. a. mismatch repair b. DNA polymerase proofreading c. nucleotide excision repair d. thymine dimers
Chromosomal DNA fragments for molecular cloning are cut by _________, and inserted into molecular vectors (such as plasmids) using __________.
a. Ligase; Restriction Endonucleases.
b. Restriction endonucleases; Ligase.
c. DNA polymerase 1; Primase.
d. DNA polymerase 1; Ligase.
e. Primase; Helicase.
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- a. Pfu Polymerase b.dNTPs c.Buffer Match each component above to the correct function(s) listed below. Write your selection(s) for each component. You may have more than one answer for each. 1. unwinds DNA 2. synthesizes new DNA strands 3. enzymatically catalyzes Quikchange 4. nucleotide source for new DNA strands 5. Energy source for reaction(s) 6. Repairs errors in base pair matching 7. Maintains pH and salt levels 8. Creates polymer chainsarrow_forward. 1 · | 2. L 3 | 4 | . 5 6 7 Direction: Encircle the letter of the correct answer . 1. It is a double-stranded , self replicating, circular DNA molecule present in bacteria which is widely used as a gene cloning vector. A. Cosmid B. Plasmid. C. Phagemid. D. Genome 2. In plant genetic engineering, which of the following acts as vector? B. Gene of interest D. Ti-plasmid А. Agrobacterium lumefaciens. Receipt plant cell. Which of the following illustrates breeding? A farmer choose a breed of cow for greater milk production The use of bacteria in order to produce human insulin. The insertion of cloned genes to plant cells. All of the above. 3. А. В. С. D. Being pest resistance is one of the traits that are being introduced to plants like corn and eggplant with the insertion of Bt-toxin gene to plant cell. What method is use when a gene is inserted to plants? A. Biolistic C. Plasmid insertion by heat shock treatment 4. B. Electroporation D. All of these 5. A biotechnologist wants to enhance…arrow_forwardn gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules _______. a. are from different organisms b. have different lengths c. have different nucleotide compositions d. have different genesarrow_forward
- Restriction enzymes cut double-stranded DNA _____. a. at noncoding areas between genes b. between bacterial and viral DNAs c. at specific base sequences d. between purines and pyrimidines e. between promoter and operator DNA sequencesarrow_forwardBacterial ____ may be used as cloning vectors. a. polymerase b. endonuclease c. plasmids d. ligase e. transcriptasearrow_forwardMatch Column A with Column B F. v Analysis of genome-wide patterns of gene expression F. v Elucidation of patterns of coordinate gene regulation A. Recombinant DNA Technology B. Sanger sequencing |derives its power from the distinctive evolutionary patterns C. Restriction Enzymes and Ligase v involves joining a donor DNA fragment of interest to a vector D. GRNA of CRISPR technology Enzymes that cleave the DNA strands E. Restriction enzymes Enzymes used in Recombinant DNA Technology F. Functional Genomics homologous to target DNA that can base pair with it The first sequencing technology that uses amplification and fluorescent dyearrow_forward
- Molecular hybridization A. Always occurs between 2 strands of DNA B. Happens at 94 degrees C C. Occurs during transformation D. Is mediated by DNA polymerase 1 E. Can be reproduced in situarrow_forwardMatch the method with the appropriate enzyme.___ PCR a. Taq polymerase___ cutting DNA b. DNA ligase___ cDNA synthesis c. reverse transcriptase___ DNA sequencing d. restriction enzyme___ pasting DNA e. DNA polymerase (not Taq)arrow_forward____ cut(s) DNA molecules at specific sites. a. DNA probes b. Restriction enzymes c. DNA polymerase d. DNA ligasearrow_forward
- The picture below depicts an aberration in the process of genetic coding. Which of the following terms best describes this occasional occurrence in genetic material? see photo attached a. Chromosomal aberration b. Mutation c. CRISPR d. ZNFarrow_forwardMatch each of the terms in the left column to the bestfitting phrase from the right column.a. exome 1. a discrete part of a protein that provides a unitof functionb. de novo gene 2. a nonfunctional member of a gene familyc. gene desert 3. the joining together of exons in a gene indifferent combinationsd. pseudogene 4. most frequent residues, either nucleotide oramino acid, found at each position in asequence alignmente. syntenic block 5. set of genes related by processes ofduplication and divergencef. orthologs 6. chromosomal region with the same genes inthe same order in two different speciesg. naturalselection7. genes with sequence similarities in twodifferent species that arose from a commonancestral geneh. consensussequence8. genes that arose by duplication within aspeciesi. gene family 9. genomic DNA sequences containing exonsj. paralogs 10. gene-poor region of the genomek. alternativeRNA splicing11. recently evolved from intergenic DNAsequencesl. protein domain 12. progressive…arrow_forwardA DNA fingerprint is based on _____. a repeating sequences found on noncoding regions b the presence of restriction enzymes c a single repeating region d repeating sequences found on coding regionsarrow_forward
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