Lab Manual for Biology
12th Edition
ISBN: 9781259298516
Author: Sylvia S. Mader Dr.
Publisher: McGraw-Hill Education
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Chapter 20.3, Problem 3CYP
Construct a hypothesis about how cyanobacteria may haveaffected the atmosphere of early Earth.
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. Consider a base substitution mutation that occurred in a DNA sequence that resulted in a change in the
encoded protein from the amino acid glutamic acid to aspartic acid. Normally the glutamic acid amino acid
is located on the outside of the soluble protein but not near an active site.
O-H¨
A. What type of mutation occurred?
O-H
B. What 2 types of chemical bonds are found in the R-groups
of each amino acid? The R groups are shaded.
CH2
CH2
CH2
H2N-C-COOH
H2N-C-COOH
1
H
Glutamic acid
H
Aspartic acid
C. What 2 types of bonds could each R-group of each of these amino acids form with other molecules?
D. Consider the chemical properties of the two amino acids and the location of the amino acid in the
protein. Explain what effect this mutation will have on this protein's function and why.
engineered constructs that consist of hollow fibers are acting as synthetic capillaries, around which cells have been loaded. The cellular space around a single fiber can be modeled as if it were a Krogh tissue cylinder. Each fiber has an outside “capillary” radius of 100 µm and the “tissue” radius can be taken as 200 µm. The following values apply to the device:R0 = 20 µM/secaO2 = 1.35 µM/mmHgDO2,T = 1.67 x 10-5 cm2/secPO2,m = 4 x 10-3 cm/secInstead of blood inside the fibers, the oxygen transport and tissue consumption are being investigated by usingan aqueous solution saturated with pure oxygen. As a result, there is no mass transfer resistance in the synthetic“capillary”, only that due to the membrane itself. Rather than accounting for pO2 variations along the length ofthe fiber, use an average value in the “capillary” of 130 mmHg.Is the tissue fully oxygenated?
Molecular Biology
Please help with question. thank you
You are studying the expression of the lac operon. You have isolated mutants as described below. In the presence of glucose, explain/describe what would happen, for each mutant, to the expression of the lac operon when you add lactose AND what would happen when the bacteria has used up all of the lactose (if the mutant is able to use lactose).5. Mutations in the lac operator that strengthen the binding of the lac repressor 200 fold
6. Mutations in the promoter that prevent binding of RNA polymerase
7. Mutations in CRP/CAP protein that prevent binding of cAMP8. Mutations in sigma factor that prevent binding of sigma to core RNA polymerase
Chapter 20 Solutions
Lab Manual for Biology
Ch. 20.1 - Prob. 1CYPCh. 20.1 - Prob. 2CYPCh. 20.1 - Prob. 3CYPCh. 20.1 - Prob. 4CYPCh. 20.2 - Explain the connection between Pasteur’s...Ch. 20.2 - Prob. 2CYPCh. 20.2 - Define three ways in which prokaryotes can...Ch. 20.3 - Prob. 1CYPCh. 20.3 - Prob. 2CYPCh. 20.3 - Construct a hypothesis about how cyanobacteria may...
Ch. 20.4 - Prob. 1CYPCh. 20.4 - Prob. 2CYPCh. 20.4 - Prob. 3CYPCh. 20 - Prob. 1NS.1QCCh. 20 - Prob. 1NS.2QCCh. 20 - Prob. 1NS.3QCCh. 20 - Prob. 2NS.1QCCh. 20 - Prob. 2NS.2QCCh. 20 - Prob. 2NS.3QCCh. 20 - Prob. 1ACh. 20 - Prob. 2ACh. 20 - Prob. 3ACh. 20 - What is the correct order of the stages in the...Ch. 20 - Prob. 5ACh. 20 - Prob. 6ACh. 20 - Prob. 7ACh. 20 - Prob. 8ACh. 20 - Prob. 9ACh. 20 - Prob. 10ACh. 20 - Prob. 11ACh. 20 - Prob. 12ACh. 20 - Prob. 13ACh. 20 - Prob. 14ACh. 20 - Prob. 15ACh. 20 - Prob. 1TSCh. 20 - Prob. 2TSCh. 20 - Prob. 3TS
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