The number of copies of target DNA after 12 cycles of polymerase chain reaction has to be calculated. Concept Introduction: Polymerase chain reaction (PCR) is widely used to amplify DNA sequences to produce millions of copies of a specific DNA segment. PCR technique finds applications for specific and rapid diagnosis of infectious diseases. The various steps involved in PCR technique is as follows: Stage 1: In the first stage, high temperature ( 94 − 98 ° C ) is provided so that DNA strands are separated and a single-stranded template gets generated. Stage 2: In the next step, annealing is done at a temperature of about 50 -65 ° C so that primer may bind to the complementary strand on the template generated in stage 1. Stage 3: In the third stage, two DNA strands obtained after annealing serve as a template for the DNA polymerase , and the enzyme then begins to extend the primers in both directions at about 72 ° C . This cycle is repeated for about 35 times until the target DNA region gets copied. Each successive cycle of a polymerases chain reaction double the gene as illustrated below:

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Chapter 20, Problem 20.87QP

Interpretation Introduction

Interpretation:

The number of copies of target DNA after 12 cycles of polymerase chain reaction has to be calculated.

Concept Introduction:

Polymerase chain reaction (PCR) is widely used to amplify DNA sequences to produce millions of copies of a specific DNA segment. PCR technique finds applications for specific and rapid diagnosis of infectious diseases.

The various steps involved in PCR technique is as follows:

Stage 1: In the first stage, high temperature (94−98 °C) is provided so that DNA strands are separated and a single-stranded template gets generated.

Stage 2: In the next step, annealing is done at a temperature of about 50-65 °C so that primer may bind to the complementary strand on the template generated in stage 1.

Stage 3: In the third stage, two DNA strands obtained after annealing serve as a template for the DNA polymerase, and the enzyme then begins to extend the primers in both directions at about 72 °C.

This cycle is repeated for about 35 times until the target DNA region gets copied.

Each successive cycle of a polymerases chain reaction double the gene as illustrated below:

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