Describe how the process of gene doning results in a cell clone contaning a recombinant plasmid.

Question

Want to see more full solutions like this?

Answer 1

Textbook Question

Chapter 20, Problem 20.1CR

Describe how the process of gene doning results in a cell clone contaning a recombinant plasmid.

Expert Solution & Answer

Summary Introduction

To explain: The gene cloning process that produces a cell clone having a recombinant plasmid.

Concept introduction:

To study a particular protein, multiple copies of the gene fragment encoding that protein are synthesized. These gene fragments are then inserted into the expression vector to synthesize a large quantity of protein that is encoded by the gene. This is known as gene cloning.

Explanation of Solution

For cloning a eukaryotic gene, the bacterial plasmid vector and the gene segment to be cloned are cleaved with the same restriction sites. Fig. 1 shows the cloning procedure using EcoRI restriction enzyme as both the sequences have their restriction sites. The arrows between the bases in Fig. 1 represent the restriction sites. The restriction digestion of the linear gene sequence and the plasmid sequence produces sticky ends. The digested gene sequence is ligated into bacterial plasmid and the recombinant plasmid is synthesized. This recombinant plasmid is reintroduced into the host cells (bacteria), where the eukaryotic gene expresses and forms proteins. The bacterial cells containing the recombinant plasmids will divide to form cell clones.

Pictorial representation:

Fig. 1 shows the cloning procedure using a gene sequence and bacterial plasmid.

CAMPBELL'S BIOLOGY MASTERING BIOLOGY, Chapter 20, Problem 20.1CR

Fig. 1 Cloning procedure

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

Humans consider themselves amazingly clever and innovative, constantly developing "new" ways of altering the world around us. As material consumption has increased, many have turned to the ideas of recycling and reuse as a means to minimize some negative aspects of our modern consumerism. Mother Nature though is the ultimate innovator and, more importantly, the ultimate recycler.

H gene assorts independently from the I gene. Both on autosomes. One man and one woman, both of HhIAIB genotype. Determine the blood type of progeny and fractions out of 16

Alleles at the P locus control seed color. Plants which are pp have white seeds, white flowers and no pigment in vegetative parts. Plants which are P_ have black seeds, purple flowers and may have varying degrees of pigment on stems and leaves. Seed color can be assessed, visually, based on if the seed is white or not white A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible. Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating. For this exercise we will use seed and data from the F10 generation of a recombinant inbred population produced using single seed…

Answer 2

Textbook Question

Chapter 20, Problem 20.1CR

Describe how the process of gene doning results in a cell clone contaning a recombinant plasmid.

Expert Solution & Answer

Summary Introduction

To explain: The gene cloning process that produces a cell clone having a recombinant plasmid.

Concept introduction:

To study a particular protein, multiple copies of the gene fragment encoding that protein are synthesized. These gene fragments are then inserted into the expression vector to synthesize a large quantity of protein that is encoded by the gene. This is known as gene cloning.

Explanation of Solution

For cloning a eukaryotic gene, the bacterial plasmid vector and the gene segment to be cloned are cleaved with the same restriction sites. Fig. 1 shows the cloning procedure using EcoRI restriction enzyme as both the sequences have their restriction sites. The arrows between the bases in Fig. 1 represent the restriction sites. The restriction digestion of the linear gene sequence and the plasmid sequence produces sticky ends. The digested gene sequence is ligated into bacterial plasmid and the recombinant plasmid is synthesized. This recombinant plasmid is reintroduced into the host cells (bacteria), where the eukaryotic gene expresses and forms proteins. The bacterial cells containing the recombinant plasmids will divide to form cell clones.

Pictorial representation:

Fig. 1 shows the cloning procedure using a gene sequence and bacterial plasmid.

CAMPBELL'S BIOLOGY MASTERING BIOLOGY, Chapter 20, Problem 20.1CR

Fig. 1 Cloning procedure

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Textbook Question

Chapter 20, Problem 20.1CR

Describe how the process of gene doning results in a cell clone contaning a recombinant plasmid.

Expert Solution & Answer

Summary Introduction

To explain: The gene cloning process that produces a cell clone having a recombinant plasmid.

Concept introduction:

To study a particular protein, multiple copies of the gene fragment encoding that protein are synthesized. These gene fragments are then inserted into the expression vector to synthesize a large quantity of protein that is encoded by the gene. This is known as gene cloning.

Explanation of Solution

For cloning a eukaryotic gene, the bacterial plasmid vector and the gene segment to be cloned are cleaved with the same restriction sites. Fig. 1 shows the cloning procedure using EcoRI restriction enzyme as both the sequences have their restriction sites. The arrows between the bases in Fig. 1 represent the restriction sites. The restriction digestion of the linear gene sequence and the plasmid sequence produces sticky ends. The digested gene sequence is ligated into bacterial plasmid and the recombinant plasmid is synthesized. This recombinant plasmid is reintroduced into the host cells (bacteria), where the eukaryotic gene expresses and forms proteins. The bacterial cells containing the recombinant plasmids will divide to form cell clones.

Pictorial representation:

Fig. 1 shows the cloning procedure using a gene sequence and bacterial plasmid.

CAMPBELL'S BIOLOGY MASTERING BIOLOGY, Chapter 20, Problem 20.1CR

Fig. 1 Cloning procedure

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Textbook Question

Chapter 20, Problem 20.1CR

Describe how the process of gene doning results in a cell clone contaning a recombinant plasmid.

Expert Solution & Answer

Summary Introduction

To explain: The gene cloning process that produces a cell clone having a recombinant plasmid.

Concept introduction:

To study a particular protein, multiple copies of the gene fragment encoding that protein are synthesized. These gene fragments are then inserted into the expression vector to synthesize a large quantity of protein that is encoded by the gene. This is known as gene cloning.

Explanation of Solution

For cloning a eukaryotic gene, the bacterial plasmid vector and the gene segment to be cloned are cleaved with the same restriction sites. Fig. 1 shows the cloning procedure using EcoRI restriction enzyme as both the sequences have their restriction sites. The arrows between the bases in Fig. 1 represent the restriction sites. The restriction digestion of the linear gene sequence and the plasmid sequence produces sticky ends. The digested gene sequence is ligated into bacterial plasmid and the recombinant plasmid is synthesized. This recombinant plasmid is reintroduced into the host cells (bacteria), where the eukaryotic gene expresses and forms proteins. The bacterial cells containing the recombinant plasmids will divide to form cell clones.

Pictorial representation:

Fig. 1 shows the cloning procedure using a gene sequence and bacterial plasmid.

CAMPBELL'S BIOLOGY MASTERING BIOLOGY, Chapter 20, Problem 20.1CR

Fig. 1 Cloning procedure

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Summary Introduction

To explain: The gene cloning process that produces a cell clone having a recombinant plasmid.

Concept introduction:

To study a particular protein, multiple copies of the gene fragment encoding that protein are synthesized. These gene fragments are then inserted into the expression vector to synthesize a large quantity of protein that is encoded by the gene. This is known as gene cloning.

Explanation of Solution

For cloning a eukaryotic gene, the bacterial plasmid vector and the gene segment to be cloned are cleaved with the same restriction sites. Fig. 1 shows the cloning procedure using EcoRI restriction enzyme as both the sequences have their restriction sites. The arrows between the bases in Fig. 1 represent the restriction sites. The restriction digestion of the linear gene sequence and the plasmid sequence produces sticky ends. The digested gene sequence is ligated into bacterial plasmid and the recombinant plasmid is synthesized. This recombinant plasmid is reintroduced into the host cells (bacteria), where the eukaryotic gene expresses and forms proteins. The bacterial cells containing the recombinant plasmids will divide to form cell clones.

Pictorial representation:

Fig. 1 shows the cloning procedure using a gene sequence and bacterial plasmid.

CAMPBELL'S BIOLOGY MASTERING BIOLOGY, Chapter 20, Problem 20.1CR

Fig. 1 Cloning procedure

Answer 8

Expert Solution & Answer

Summary Introduction

To explain: The gene cloning process that produces a cell clone having a recombinant plasmid.

Concept introduction:

To study a particular protein, multiple copies of the gene fragment encoding that protein are synthesized. These gene fragments are then inserted into the expression vector to synthesize a large quantity of protein that is encoded by the gene. This is known as gene cloning.

Explanation of Solution

For cloning a eukaryotic gene, the bacterial plasmid vector and the gene segment to be cloned are cleaved with the same restriction sites. Fig. 1 shows the cloning procedure using EcoRI restriction enzyme as both the sequences have their restriction sites. The arrows between the bases in Fig. 1 represent the restriction sites. The restriction digestion of the linear gene sequence and the plasmid sequence produces sticky ends. The digested gene sequence is ligated into bacterial plasmid and the recombinant plasmid is synthesized. This recombinant plasmid is reintroduced into the host cells (bacteria), where the eukaryotic gene expresses and forms proteins. The bacterial cells containing the recombinant plasmids will divide to form cell clones.

Pictorial representation:

Fig. 1 shows the cloning procedure using a gene sequence and bacterial plasmid.

CAMPBELL'S BIOLOGY MASTERING BIOLOGY, Chapter 20, Problem 20.1CR

Fig. 1 Cloning procedure

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

To explain: The gene cloning process that produces a cell clone having a recombinant plasmid.

Concept introduction:

To study a particular protein, multiple copies of the gene fragment encoding that protein are synthesized. These gene fragments are then inserted into the expression vector to synthesize a large quantity of protein that is encoded by the gene. This is known as gene cloning.

Answer 12

Explanation of Solution

For cloning a eukaryotic gene, the bacterial plasmid vector and the gene segment to be cloned are cleaved with the same restriction sites. Fig. 1 shows the cloning procedure using EcoRI restriction enzyme as both the sequences have their restriction sites. The arrows between the bases in Fig. 1 represent the restriction sites. The restriction digestion of the linear gene sequence and the plasmid sequence produces sticky ends. The digested gene sequence is ligated into bacterial plasmid and the recombinant plasmid is synthesized. This recombinant plasmid is reintroduced into the host cells (bacteria), where the eukaryotic gene expresses and forms proteins. The bacterial cells containing the recombinant plasmids will divide to form cell clones.

Pictorial representation:

Fig. 1 shows the cloning procedure using a gene sequence and bacterial plasmid.

CAMPBELL'S BIOLOGY MASTERING BIOLOGY, Chapter 20, Problem 20.1CR

Fig. 1 Cloning procedure