Chapter 20, Problem 13PIAT

Summary Introduction

To review:

The blank space given in a statement, “If the sequence of DNA in question 12 were amplified using 25 PCR cycles, then the amount of this DNA would be predicted to increase by 33.6 million or __________fold.”

Introduction:

PCR is the technique used to amplify or produce multiple copies of the DNA fragments. Every cycle of PCR doubles the amount of DNA.

Transgenic plants usually contain genes of bacterial plasmid origin. In a study, researchers transformed cells from a susceptible potato variety with a potato blight resistance gene cloned from a resistant variety. They performed PCR using primers specific for the plasmid to determine which plants from this group were also free of plasmid DNA (cloning vector) sequences. The positive control lane shows PCR amplification of plasmid DNA only, and the negative control lane shows an attempted PCR amplification of no added DNA.