The blank space given in a statement, “If the sequence of DNA in question 12 were amplified using 25 PCR cycles, then the amount of this DNA would be predicted to increase by 33.6 million or __________fold.” Introduction: PCR is the technique used to amplify or produce multiple copies of the DNA fragments. Every cycle of PCR doubles the amount of DNA. Transgenic plants usually contain genes of bacterial plasmid origin. In a study, researchers transformed cells from a susceptible potato variety with a potato blight resistance gene cloned from a resistant variety. They performed PCR using primers specific for the plasmid to determine which plants from this group were also free of plasmid DNA (cloning vector) sequences. The positive control lane shows PCR amplification of plasmid DNA only, and the negative control lane shows an attempted PCR amplification of no added DNA.

Question

Want to see the full answer?

Answer 1

Question

Chapter 20, Problem 13PIAT

Summary Introduction

To review:

The blank space given in a statement, “If the sequence of DNA in question 12 were amplified using 25 PCR cycles, then the amount of this DNA would be predicted to increase by 33.6 million or __________fold.”

Introduction:

PCR is the technique used to amplify or produce multiple copies of the DNA fragments. Every cycle of PCR doubles the amount of DNA.

Transgenic plants usually contain genes of bacterial plasmid origin. In a study, researchers transformed cells from a susceptible potato variety with a potato blight resistance gene cloned from a resistant variety. They performed PCR using primers specific for the plasmid to determine which plants from this group were also free of plasmid DNA (cloning vector) sequences. The positive control lane shows PCR amplification of plasmid DNA only, and the negative control lane shows an attempted PCR amplification of no added DNA.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

4

Does it show the level of proteins? What about the amount? Levels of protein activation? How can you tell? Does the thickness tell you anything? What about the number of the lines? And the other questions

KD 200- 116- 66- Vec ATF6 (670) ATF6 (402) ATF6 (373) ATF6 (366) I I 45- 1 2 3 4 5 ATFG (360) (e/c) 9V ATFG (402) g ant- ATF anti-KDEL DAPI b

Answer 2

Question

Chapter 20, Problem 13PIAT

Summary Introduction

To review:

The blank space given in a statement, “If the sequence of DNA in question 12 were amplified using 25 PCR cycles, then the amount of this DNA would be predicted to increase by 33.6 million or __________fold.”

Introduction:

PCR is the technique used to amplify or produce multiple copies of the DNA fragments. Every cycle of PCR doubles the amount of DNA.

Transgenic plants usually contain genes of bacterial plasmid origin. In a study, researchers transformed cells from a susceptible potato variety with a potato blight resistance gene cloned from a resistant variety. They performed PCR using primers specific for the plasmid to determine which plants from this group were also free of plasmid DNA (cloning vector) sequences. The positive control lane shows PCR amplification of plasmid DNA only, and the negative control lane shows an attempted PCR amplification of no added DNA.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 20, Problem 13PIAT

Summary Introduction

To review:

The blank space given in a statement, “If the sequence of DNA in question 12 were amplified using 25 PCR cycles, then the amount of this DNA would be predicted to increase by 33.6 million or __________fold.”

Introduction:

PCR is the technique used to amplify or produce multiple copies of the DNA fragments. Every cycle of PCR doubles the amount of DNA.

Transgenic plants usually contain genes of bacterial plasmid origin. In a study, researchers transformed cells from a susceptible potato variety with a potato blight resistance gene cloned from a resistant variety. They performed PCR using primers specific for the plasmid to determine which plants from this group were also free of plasmid DNA (cloning vector) sequences. The positive control lane shows PCR amplification of plasmid DNA only, and the negative control lane shows an attempted PCR amplification of no added DNA.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 20, Problem 13PIAT

Summary Introduction

To review:

The blank space given in a statement, “If the sequence of DNA in question 12 were amplified using 25 PCR cycles, then the amount of this DNA would be predicted to increase by 33.6 million or __________fold.”

Introduction:

PCR is the technique used to amplify or produce multiple copies of the DNA fragments. Every cycle of PCR doubles the amount of DNA.

Transgenic plants usually contain genes of bacterial plasmid origin. In a study, researchers transformed cells from a susceptible potato variety with a potato blight resistance gene cloned from a resistant variety. They performed PCR using primers specific for the plasmid to determine which plants from this group were also free of plasmid DNA (cloning vector) sequences. The positive control lane shows PCR amplification of plasmid DNA only, and the negative control lane shows an attempted PCR amplification of no added DNA.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 20, Problem 13PIAT

Summary Introduction

To review:

The blank space given in a statement, “If the sequence of DNA in question 12 were amplified using 25 PCR cycles, then the amount of this DNA would be predicted to increase by 33.6 million or __________fold.”

Introduction:

PCR is the technique used to amplify or produce multiple copies of the DNA fragments. Every cycle of PCR doubles the amount of DNA.

Transgenic plants usually contain genes of bacterial plasmid origin. In a study, researchers transformed cells from a susceptible potato variety with a potato blight resistance gene cloned from a resistant variety. They performed PCR using primers specific for the plasmid to determine which plants from this group were also free of plasmid DNA (cloning vector) sequences. The positive control lane shows PCR amplification of plasmid DNA only, and the negative control lane shows an attempted PCR amplification of no added DNA.

Answer 6

Chapter 20, Problem 13PIAT

Summary Introduction

To review:

The blank space given in a statement, “If the sequence of DNA in question 12 were amplified using 25 PCR cycles, then the amount of this DNA would be predicted to increase by 33.6 million or __________fold.”

Introduction:

PCR is the technique used to amplify or produce multiple copies of the DNA fragments. Every cycle of PCR doubles the amount of DNA.

Transgenic plants usually contain genes of bacterial plasmid origin. In a study, researchers transformed cells from a susceptible potato variety with a potato blight resistance gene cloned from a resistant variety. They performed PCR using primers specific for the plasmid to determine which plants from this group were also free of plasmid DNA (cloning vector) sequences. The positive control lane shows PCR amplification of plasmid DNA only, and the negative control lane shows an attempted PCR amplification of no added DNA.

Answer 7

Chapter 20, Problem 13PIAT

Summary Introduction

To review:

The blank space given in a statement, “If the sequence of DNA in question 12 were amplified using 25 PCR cycles, then the amount of this DNA would be predicted to increase by 33.6 million or __________fold.”

Introduction:

PCR is the technique used to amplify or produce multiple copies of the DNA fragments. Every cycle of PCR doubles the amount of DNA.

Transgenic plants usually contain genes of bacterial plasmid origin. In a study, researchers transformed cells from a susceptible potato variety with a potato blight resistance gene cloned from a resistant variety. They performed PCR using primers specific for the plasmid to determine which plants from this group were also free of plasmid DNA (cloning vector) sequences. The positive control lane shows PCR amplification of plasmid DNA only, and the negative control lane shows an attempted PCR amplification of no added DNA.

Answer 8

Expert Solution & Answer

Answer 9

Expert Solution & Answer

Answer 10

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 11

Ch. 20 - Prob. 1TYK Ch. 20 - What is a plasmid? a. an organelle found in many...Ch. 20 - 3. Once the gene that causes Huntington disease...Ch. 20 - CAUTION The human genome size is 3 billion base...Ch. 20 - Prob. 5TYU Ch. 20 - Prob. 6TYU Ch. 20 - Prob. 7TYU Ch. 20 - Prob. 8TYU Ch. 20 - 9. Revolutionaries executed Nicholas II, the last...Ch. 20 - Prob. 10TYPSS

Solution Summary: The author explains PCR is the technique used to amplify or produce multiple copies of the DNA fragments. Transgenic plants contain genes of bacterial plasmid origin.

Concept explainers

Videos

Want to see the full answer?

Chapter 20 Solutions