Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 2, Problem 1Q
What, if any, practical value do these techniques have?
Expert Solution & Answer
Summary Introduction
To analyze:
The practical values of these techniques.
Introduction:
Mounting is the procedure followed for observing a specimen under the microscope successfully. It includes placing the microorganism on glass slide, placing an immersion liquid (not always) and covering it with a coverslip that helps in holding the specimen at a place, equal distribution of any immersion liquid( if used), protection of specimen from accidental contact or dust and preventing contact of specimen and objective lens of the microscope.
Explanation of Solution
Mounting media is generally used for embedding and highlight the specimen during mounting. A good mounting media have properties like a similar refractive index as that of a glass slide (reduces the refraction of light), stability, solubility in the medium in which the specimen has been prepared, avoid fading of specimen stain and it should not react with the specimen.
A proper mounting of a specimen helps in observing the shape, size, and motility of the microorganism. It also helps in identifying the specimen under observation.
Conclusion
Practical values of mounting include identification of specimens and observing its motility, size, and shape.
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Chapter 2 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 2 - What, if any, practical value do these techniques...Ch. 2 - Prob. 2QCh. 2 - Why is petroleum jelly used in the hanging-drop...Ch. 2 - Why are microorganisms hard to see in wet...Ch. 2 - Can you distinguish the prokaryotic organisms from...Ch. 2 - Why isnt the oil immersion lens used in the...Ch. 2 - Where did the organisms in the infusions come...Ch. 2 - A wet mount may be used for quick examination of...
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