Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 2, Problem 1Q
What, if any, practical value do these techniques have?
Expert Solution & Answer
Summary Introduction
To analyze:
The practical values of these techniques.
Introduction:
Mounting is the procedure followed for observing a specimen under the microscope successfully. It includes placing the microorganism on glass slide, placing an immersion liquid (not always) and covering it with a coverslip that helps in holding the specimen at a place, equal distribution of any immersion liquid( if used), protection of specimen from accidental contact or dust and preventing contact of specimen and objective lens of the microscope.
Explanation of Solution
Mounting media is generally used for embedding and highlight the specimen during mounting. A good mounting media have properties like a similar refractive index as that of a glass slide (reduces the refraction of light), stability, solubility in the medium in which the specimen has been prepared, avoid fading of specimen stain and it should not react with the specimen.
A proper mounting of a specimen helps in observing the shape, size, and motility of the microorganism. It also helps in identifying the specimen under observation.
Conclusion
Practical values of mounting include identification of specimens and observing its motility, size, and shape.
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9. Aerobic respiration of one lipid molecule. The lipid is composed of one glycerol molecule connected to two
fatty acid tails. One fatty acid is 12 carbons long and the other fatty acid is 18 carbons long in the figure
below. Use the information below to determine how much ATP will be produced from the glycerol part of
the lipid. Then, in part B, determine how much ATP is produced from the 2 fatty acids of the lipid. Finally
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total number of ATP produced per lipid. Assume no other carbon source is available.
18 carbons
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pathway shown in the figure below. Notice this process costs one ATP but generates one FADH2. Continue
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Chapter 2 Solutions
Laboratory Experiments in Microbiology (11th Edition)
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