Chapter 2, Problem 1MIR

You suspect a protein to be secreted out of a cell. What experiment would you perform to follow the movement of this protein through the secretory pathway? A. Pulse - Chase Experiment OB. Signal Sequence Deletion C. Signal Sequence Mutation OD. All of the above E. None of the above

A schematic drawing of the secretory and endocytic pathways is presented below. Indicate which component in the drawing (A to J) corresponds to each of the following. Your answer would be a 10-letter string composed of letters A to J only, c.g. HICDJABFGE. nuclear envelope Early endosome Late endosome ER Lysosome A ( ) cis Golgi cisterna () modial Golgi cisterna () trans Golgi cisterna [] cis Golgi network (CGN) () trans Golgi network (TGN) () Secretory Vesicle B C D E CYTOSOL F G H plasma membrane

Which of the following guide transport vesicles to their target membrane? A. Rab monomeric GTPases B. Signal Sequence OC. Heat Shock Proteins OD. All of the above O E. None of the above

Stroma Light 4 H 4 H I Thylakoid- membran 2 H₂O D1 D2 Min Light 2,6 ATP 2 NADP+ 2 NADPH/H+ Fp 2,6 ADP +2,6 P 8 H+ 12 H+ 4 H+ Lumen PS II Cyt bef PS I ATP-Synthase 12345 5a 5b 6

Which of the following promote membrane fusion between the transport vesicle and the target compartment? OA. Endocytosis OB. t-SNARE OC. v-SNARE OD. Both B and C O E. None of the above

The following diagram depicts the topology of a multipass transmembrane protein in the endoplasmic reticulum (ER) membrane. Which set of helices act Cytosol N C 1 2 as stop-transfer signals in this protein? 2 3 4 5 6 OA 1,2,3 OB. 4. 5. 6 OC. 1.3.5 D. 2, 4, 6 OE1.6 ER lumen

A signal peptidase cleaves off the signal sequence upon import of the protein into the mitochondrial matrix. Where is this signal peptidase located? A. Outer membrane OB. Inner membrane C. Mitochondrial Matrix OD. A and B OE. None of the above

Stroma Light 4 H 4 H I Thylakoid- membran 2 H₂O D1 D2 Min Light 2,6 ATP 2 NADP+ 2 NADPH/H+ Fp 2,6 ADP +2,6 P 8 H+ 12 H+ 4 H+ Lumen PS II Cyt bef PS I ATP-Synthase 12345 5a 5b 6

Which of the following complexes is responsible for bringing Nucleus encoded proteins into the mitochondrial matrix? OA. TOM Complex OB. TIM 23 Complex OC. Both A and B OD. OXA complex E. None of the above