BIOLOGY
BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 2, Problem 1DA

Data analysis If we call each step on the x-axis one volume of base, how many volumes of base must be added to change the pH from 4 to 6?

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Summary Introduction

To determine: The volumes of the base need to add on the x-axis to change the pH from 4 to 6.

Introduction: Buffer solution is an aqueous solution that contains a solution of a weak acid and connected base. All living cells and fluid present in the multicellular organism contain a neutral pH.

Explanation of Solution

The basicity of the solution depends upon the ion concentration of H+ ions and OH- ions. Every basic solution has a pH value above 7. The range of basic solution in pH scale lies above 7 till 14. A basic solution is required to neutralize the acid present in the solution that helps to increase the pH. Every step on the x-axis consisting of one volume of base. Therefore, the pH change from 4 to 6 on the x-axis is required two-volume of the base.

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Question: Which base (A, C, T or G) corresponds to X in the unknown? I did the experiment, got the data below, and calculated the binding constants.  But I am TOTALLY lost as to how to figure this out! I don't even know what steps I would take.   Base pairs Data – all had Temp = 250C PH = 7 Binding Constant A & X [A] = 0.00373221M [X] = 0.00373221M [AX] = 0.0462678M 3.322 C & X [C] = 0.0469007M [X] = 0.0469007M [CX] = 0.00309935M 1.409 T & X [T] = 0.0452279M [X] = 0.0452279M [TX] = 0.00477212M 2.333 G & X [G] = 0.0469554M [X] = 0.0469554M [GX] = 0.00304456M 2.633
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GCSE Chemistry - Acids and Bases #34; Author: Cognito;https://www.youtube.com/watch?v=vt8fB3MFzLk;License: Standard youtube license