Chapter 2, Problem 17P

Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.

Plot the Titration Curve for Bicine and Calculate How to Prepare a pH 7.5 Bicine Buffer Solution Bicine (N, N—bis (2-hydroxyethyl) glycine) is another commonly used buffer in biochemistry labs. The structure of Bicine in its fully protonated form is shown here:

1. Draw the titration curve for Bicine. assuming the pA'_a for its free COOH group is 2.3 and the pA_a for its tertiary amino group is 8.3.
2. Draw the structure of the fully deprotonated form (completely dissociated form) of bicine.
3. You have available a U.l Msolution of Bicine at its isoelectric point (pH|)_T 0.1 M solutions of HCI and NaOH. and ample distilled water. Describe the preparation of 1 L of 0.U4 M Bicine buffer. pH 7.5.
4. What is the concentration of the fully protonated form of Bicine in your final buffer solution?