BIOLOGY-TEXT
BIOLOGY-TEXT
5th Edition
ISBN: 9781260169621
Author: BROOKER
Publisher: MCG
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Chapter 19.5, Problem 3CC

Gene Transfer Between Bacteria

Concept Check: Is transduction a normal part of the phage life cycle? Explain.

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Generalized transduction can transfer:   please explain the answer a.any DNA from the host genome b.only phage-specific DNA sequences c.genes flanked by insertion elements d.only genes near the site of integration
Based on the phage three factor info. What were the phenotypes of the parental phage strains used in this experiment?  After performing your cross, what percent of the plaques from the progeny phage would you expect to be cloudy? After performing your cross, what percent of the plaques from the progeny phage would you expect to have the parental genotypes?
About the technique of phage display: MOLECULAR BIOLOGY_advanced The Escherichia coli cell infected by the phage codifies for the optimized ligand when the phage DNA integrates in the DNA of the bacteria. Phages are selected if they express on their surface the optimized ligand. One selects Escherichia coli cells that are resistant to the phage infection. More than one optimized ligand can be selected during the panning procedure. The ligand to be selected on the surface of the phage is non-covalently linked to one of the surface proteins.
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