Chapter 19, Problem 9MCP

Interpretation Introduction

Interpretation:

The reason behind the selective inhibition of chymotrypsin and not trypsin by $\text{L-1-}\left(\text{p-toluenesulfonyl}\right)\text{-amido-2-phenylethylchloromethyl ketone }$ has to be determined.

Concept Introduction:

Chymotrypsin cleaves at the carboxyl-terminal of aromatic amino acids such as phenylalanine, tyrosine, and tryptophan. Trypsin cleaves at the carboxyl-terminal of positively charged amino acid lysine and arginine.

The binding site of chymotrypsin is illustrated as follows: