EXPLOR. CHEM ANALYSIS (LL) - W/ ACHIEVE
EXPLOR. CHEM ANALYSIS (LL) - W/ ACHIEVE
5th Edition
ISBN: 9781319468859
Author: Harris
Publisher: MAC HIGHER
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Chapter 19, Problem 19.21P
Interpretation Introduction

Interpretation:

Amplification of analyte response as done in enzyme-linked immunoassay technique has to be explained.

Concept Introduction:

Immunoassay is important biochemical tool that can measure and quantify the concentration of small molecule. Immunoassay is often supported by antibodies called antigens. ELISA is one such enzyme-linked immunoassay technique.

In ELISA technique certain target macromolecule called antigen is immobilized and fixed to surface of microplate. This then forms complex with reporter enzyme and detection is accomplished by measurement of reporter enzyme’s activity via incubation to produce significant product. Essential feature of this method is extremely specific antigen-antibody interaction.

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Predict the products of this organic reaction: H3O+ + ? • Draw all the reasonable products in the drawing area below. If there are no products, because no reaction will occur, check the box under the drawing area. • Include both major and minor products, if some of the products will be more common than others. • Be sure to use wedge and dash bonds if you need to distinguish between enantiomers. No reaction. Click and drag to start drawing a structure. dm
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