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Chapter 19, Problem 12P
Summary Introduction

To explain: The rate at which flight muscles of Lucilia sericata turns over ATP pool.

Introduction:

Almost exclusively, the ATP (adenosine triphosphate) is produced from oxidative phosphorylation in flight muscle of Lucilia sericata. The flight muscle of the fly makes up around 20% of the weight of fly.

Summary Introduction

To explain:

The time up to which the reservoir would last in absence of oxidative phosphorylation.

Introduction:

ATP is also referred to as currency of cell and is involved in the energy transfer. It provides energy to the chemical reaction and drives them in favorable direction.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism  of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
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