Chapter 17, Problem 8P

Summary Introduction

To explain: The definite activity of the isolated acetate is $\text{1}\text{.00}\times {\text{10}}^{\text{7}}\text{ cpm/μmol}$. Whether the result obtained is consistent with the beta oxidation pathway.

Introduction:

The nucleus of tritium ( $\text{H}$) which is a hydrogen’s radioactive isotope, consist of one proton along with two neutrons. Palmitate a saturated fatty acid labeled with tritium to a particular activity of $\text{2}\text{.48}\times {\text{10}}^8\text{ cpm}$ of palmitate is added to mitochondrial and undergoes beta oxidation to form acetyl-coenzyme A. The isolated and hydrolyzed acetate from acetyl-coenzyme A show the specific activity of $\text{1}\text{.00}\times {\text{10}}^{\text{7}}\text{ cpm/μmol}$.

Summary Introduction

To explain: The final fate of the removed tritium.

Introduction:

Palmitate a saturated fatty acid labeled with tritium to a specific activity of $\text{2}\text{.48}\times {\text{10}}^8\text{ cpm}$ of palmitate is added to mitochondrial and undergoes beta oxidation to form acetyl-coenzyme A. The isolated and hydrolyzed acetate from acetyl-coenzyme A show the specific activity of $\text{1}\text{.00}\times {\text{10}}^{\text{7}}\text{ cpm/μmol}$.