Essential Cell Biology
Essential Cell Biology
4th Edition
ISBN: 9780815344544
Author: Keith Roberts, Karen Hopkin, Alexander D Johnson, Martin Raff, Dennis Bray, Bruce Alberts, Julian Lewis, Peter Walter
Publisher: TAYLOR
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Chapter 17, Problem 3Q
Summary Introduction

A.

To explain: The changes at the end of shrinking phase microtubule in order to stop its shrinking and to start growing again.

Introduction: Dynamic instability is characterized by interchange between phases of relatively slow growing and rapid shortening at the ends of individual microtubules. It is a result of the hydrolysis of guanosine triphosphate (GTP)-bound to β-tubulin shortly after assembly. The microtubules grow rapidly when GTP caps are present in its end, whereas the microtubules that are associated with guanosine diphosphate (GDP)-tubulin heterodimers at their ends are rapidly depolymerized.

B.

Summary Introduction

To explain: How the changes in the tubulin concentration affect the shrinking phase microtubule.

Introduction: Dynamic instability is characterized by interchange between phases of relatively slow growing and rapid shortening at the ends of individual microtubules. It is a result of the hydrolysis of guanosine triphosphate (GTP)-bound to β-tubulin shortly after assembly. The microtubules grow rapidly when GTP caps are present in its end, whereas the microtubules that are associated with guanosine diphosphate (GDP)-tubulin heterodimers at their ends are rapidly depolymerized.

C.

Summary Introduction

To explain: The results when the solution contained only GDP and not GTP.

Introduction: Dynamic instability is characterized by interchange between phases of relatively slow growing and rapid shortening at the ends of individual microtubules. It is a result of the hydrolysis of guanosine triphosphate (GTP)-bound to β-tubulin shortly after assembly. The microtubules grow rapidly when GTP caps are present in its end, whereas the microtubules that are associated with guanosine diphosphate (GDP)-tubulin heterodimers at their ends are rapidly depolymerized.

D.

Summary Introduction

To explain: The results if the solution contains an analog GTP that cannot be hydrolyzed.

Introduction: Dynamic instability is characterized by interchange between phases of relatively slow growing and rapid shortening at the ends of individual microtubules. It is a result of the hydrolysis of guanosine triphosphate (GTP)-bound to β-tubulin shortly after assembly. The microtubules grow rapidly when GTP caps are present in its end, whereas the microtubules that are associated with guanosine diphosphate (GDP)-tubulin heterodimers at their ends are rapidly depolymerized.

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