1 SEM ACC W/RAVEN CARDED
12th Edition
ISBN: 9781265486297
Author: Raven
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 17, Problem 2A
Which of the following statements is accurate for
a. DNA primers are required.
b. DNA polymerase is stable at high temperatures.
c. Ligase is essential.
d. dNTPs are necessary.
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Polymerase Chain Reaction, or PCR, can
Group of answer choices
A. target a specific region of DNA and cut it out of the rest of the genetic material for further analysis.
B. copy the number of copies of a selected region of DNA linearly.
C. increase the number of copies of a selected region of DNA exponentially.
D. copy the entire genome at least a dozen times.
Which of the following is NOT true of DNA polymerase?
a. DNA polymerase builds the new strand from 5' to 3'.
b. DNA polymerase can start new DNA strands independently.
c. DNA polymerase uses a "guess and check" method to add nucleotides.
d. DNA polymerase uses dATP as a nucleotide.
e. DNA polymerase reads the template from 3' to 5'.
Which of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR?
A. PCR is more likely to have errors introduced in the copying process.
B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR.
C. PCR can only be conducted in eukaryotic cells.
D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.
Chapter 17 Solutions
1 SEM ACC W/RAVEN CARDED
Ch. 17.1 - Prob. 1LOCh. 17.1 - Prob. 2LOCh. 17.1 - Describe the construction and uses of recombinant...Ch. 17.2 - Relate the process of DNA replication to PCR.Ch. 17.2 - Compare and contrast PCR, RT-PCR, and quantitative...Ch. 17.3 - Prob. 1LOCh. 17.3 - Prob. 2LOCh. 17.3 - Describe the pros and cons of RNA interference and...Ch. 17.4 - Explain how the universal nature of the genetic...Ch. 17.4 - Compare and contrast knockout, knockin, and...
Ch. 17.4 - Prob. 3LOCh. 17.5 - Describe the benefits of biofuel production from...Ch. 17.5 - Prob. 2LOCh. 17.5 - Prob. 3LOCh. 17.6 - Prob. 1LOCh. 17.6 - Compare and contrast FISH and gene chip...Ch. 17.6 - Describe how immunoassays can be used to diagnose...Ch. 17.7 - Describe the benefits of creating transgenic...Ch. 17.7 - Prob. 2LOCh. 17.7 - Evaluate issues on each side of the transgenic...Ch. 17 - Prob. 1DACh. 17 - Prob. 2DACh. 17 - Prob. 1IQCh. 17 - Prob. 2IQCh. 17 - You study a gene known to be important in the...Ch. 17 - What is the basis of separation of different DNA...Ch. 17 - Prob. 3UCh. 17 - FISH analysis of a breast tumor biopsy for HER2...Ch. 17 - In terms of studying gene function, what is the...Ch. 17 - The Ti plasmid of Agrobacterium usually induces...Ch. 17 - Prob. 1ACh. 17 - Which of the following statements is accurate for...Ch. 17 - Prob. 3ACh. 17 - Many human proteins, such as hemoglobin, are only...Ch. 17 - Amyloid beta is a proteolytic product of a protein...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Which of the following is FALSE about current Sanger dideoxy DNA sequencing procedures? a. Chain termination occurs during synthesis of a new DNA strand. b. Many steps can be automated. c. No DNA is synthesized in the procedure. d. Fluorescent molecules can be used to detect the DNA.arrow_forwardMatch the terms associated with the polymerase chain reaction with their correct descriptions. Refers to the fact that DNA molecules get longer the more of them there are in the reaction. A. В. Heat the sample to a high temperature (usually 94°C) to separate all DNA strands from each other. Denaturation C. Incubate the reaction at the optimal temperature for the primers to base-pair with each other. Annealing. D. Incubate at a low enough temperature (usually-55°C) so that primers base-pair with their complementary sequence. Extension. Add a chaotropic agent that destabilizes hydrogen bonding. E. Amplification. F. Incubate the sample at a temperature that is optimal for thermostable Taq DNA polymerase (usually -72°C). G. Happens after repeated cycles of the temperature change regimen. Refers to the quadrupling of the target DNA sequence in every cycle of the temperature regimen. Н.arrow_forwardPredict the effect on the PCR reaction if any of the following circumstances arose: i) there are no primers in the reaction, i) there are no dNTPs in the reaction, iii) there is no Taq polymerase in the reaction, A PCR would not commence. B. PCR would proceed normally. C. PCR would cease after a few cycles. D. Non-specific DNA amplification will occur.arrow_forward
- Which of the following statements about DNA probes are false? a. Probes can be labeled with a fluorescent dye to facilitate detection. b. Probes can be labeled with radioactive phosphorus to facilitate detection. c. Probes are typically composed of double stranded pieces of DNA. d. Probes are typically composed of single stranded pieces of DNA.arrow_forwardWhat would be the effect of performing a RT-PCR with the following ingredients: an mRNA template, appropriate primers, dNTPs, heat-stable reverse transcriptase and human DNA polymerase? Select one: a. The PCR would occur, but with a high mutation rate b. The PCR reaction will not commence c. Non-specific PCR of random templates will occur d. The RNA template would be converted to DNA, but the DNA segment would not be amplified PCR would proceed normally е.arrow_forwardWhat would be the outcome if the primers used in a polymerase chain reaction have lower GC content (<40 %), shorter, and more variable than the intended oligonucleotide sequence? a.The PCR reaction will cease after the first cycle b.The reaction will yield a mixture of non-specific products. c.All of these d.The PCR reaction will not start. e.The reaction will yield a single short PCR product.arrow_forward
- Which statement about Okazaki fragments is true? Select one: a. DNA polymerase doesn’t need a primer to build these fragments b. They act as a primer that initiates DNA replication. c. They correct errors made during earlier phases of DNA replication. d. They are necessary because DNA polymerase can only build DNA in the 5’ to 3’ direction, so for one of the strands at each fork, the DNA polymerase can only buildaway from the fork. e. They prevent the ends of chromosomes from shortening with every replication.arrow_forwardWhich of the following correctly matches the step of polymerase chain reaction (PCR) with its events? A. Extension - reaction is cooled and primers bind to complementary sequences on template DNA. B. Denaturation - high temperature separates the DNA C. Annealing - Taq polymerase extends the primers, synthesizing DNAarrow_forwardPlace the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.arrow_forward
- PCR is used to: Select one: a. Randomly break large pieces of DNA or plasmids into smaller fragments. O b. Determine if a particular piece of DNA contains a certain gene. Oc. Amplify a specific segment of DNA. d. Separate DNA fragments based on size. e. Isolate specific pieces of DNA. ess Jump to...arrow_forwardDNA polymerases make errors in matching as DNA is synthesized. These errors can be detected and repaired by: A. Ligase B. DNA polymerase C. Primase D. Helicasearrow_forwardIdentify the CORRECT sequence of steps that occurs during every cycle of PCR. I. The primers hybridize to the target DNA. II. Binding of DNA polymerase to the target DNA. UI. Extension of primers to make a copy of the target DNA. IV. The mixture is heated to a high temperature to denature the double stranded target DNA. A. I, II, III, IV. B. II, I, IV, II. C. IV, I, II, II. D. IV, II, I, III.arrow_forward
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