The way in which a strain of yeast is used to identify the prey proteins along with the ways in which this protocol is relevant to the goal of finding the proteins. Introduction: The yeast two-hybrid system method has three components. First: the yeast that contains a reporter gene construct in which UASG drives the expression of an E.Coli . Second: the yeast expresses the fusion protein in which the DNA binding domain of GAL4 is fused to the protein of interest. Third: a cDNA library made in plasmids where each cDNA is fused in frame to the activation domain of GAL4.

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Answer 1

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Chapter 17, Problem 17P

Summary Introduction

To determine:

The way in which a strain of yeast is used to identify the prey proteins along with the ways in which this protocol is relevant to the goal of finding the proteins.

Introduction:

The yeast two-hybrid system method has three components. First: the yeast that contains a reporter gene construct in which UASG drives the expression of an E.Coli. Second: the yeast expresses the fusion protein in which the DNA binding domain of GAL4 is fused to the protein of interest. Third: a cDNA library made in plasmids where each cDNA is fused in frame to the activation domain of GAL4.

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Answer 2

Question

Chapter 17, Problem 17P

Summary Introduction

To determine:

The way in which a strain of yeast is used to identify the prey proteins along with the ways in which this protocol is relevant to the goal of finding the proteins.

Introduction:

The yeast two-hybrid system method has three components. First: the yeast that contains a reporter gene construct in which UASG drives the expression of an E.Coli. Second: the yeast expresses the fusion protein in which the DNA binding domain of GAL4 is fused to the protein of interest. Third: a cDNA library made in plasmids where each cDNA is fused in frame to the activation domain of GAL4.

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Answer 3

Question

Chapter 17, Problem 17P

Summary Introduction

To determine:

The way in which a strain of yeast is used to identify the prey proteins along with the ways in which this protocol is relevant to the goal of finding the proteins.

Introduction:

The yeast two-hybrid system method has three components. First: the yeast that contains a reporter gene construct in which UASG drives the expression of an E.Coli. Second: the yeast expresses the fusion protein in which the DNA binding domain of GAL4 is fused to the protein of interest. Third: a cDNA library made in plasmids where each cDNA is fused in frame to the activation domain of GAL4.

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Answer 4

Question

Chapter 17, Problem 17P

Summary Introduction

To determine:

The way in which a strain of yeast is used to identify the prey proteins along with the ways in which this protocol is relevant to the goal of finding the proteins.

Introduction:

The yeast two-hybrid system method has three components. First: the yeast that contains a reporter gene construct in which UASG drives the expression of an E.Coli. Second: the yeast expresses the fusion protein in which the DNA binding domain of GAL4 is fused to the protein of interest. Third: a cDNA library made in plasmids where each cDNA is fused in frame to the activation domain of GAL4.

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Answer 5

Chapter 17, Problem 17P

Summary Introduction

To determine:

The way in which a strain of yeast is used to identify the prey proteins along with the ways in which this protocol is relevant to the goal of finding the proteins.

Introduction:

The yeast two-hybrid system method has three components. First: the yeast that contains a reporter gene construct in which UASG drives the expression of an E.Coli. Second: the yeast expresses the fusion protein in which the DNA binding domain of GAL4 is fused to the protein of interest. Third: a cDNA library made in plasmids where each cDNA is fused in frame to the activation domain of GAL4.

Answer 6

Chapter 17, Problem 17P

Summary Introduction

To determine:

The way in which a strain of yeast is used to identify the prey proteins along with the ways in which this protocol is relevant to the goal of finding the proteins.

Introduction:

The yeast two-hybrid system method has three components. First: the yeast that contains a reporter gene construct in which UASG drives the expression of an E.Coli. Second: the yeast expresses the fusion protein in which the DNA binding domain of GAL4 is fused to the protein of interest. Third: a cDNA library made in plasmids where each cDNA is fused in frame to the activation domain of GAL4.

Answer 7

Chapter 17, Problem 17P

Summary Introduction

To determine:

The way in which a strain of yeast is used to identify the prey proteins along with the ways in which this protocol is relevant to the goal of finding the proteins.

Introduction:

The yeast two-hybrid system method has three components. First: the yeast that contains a reporter gene construct in which UASG drives the expression of an E.Coli. Second: the yeast expresses the fusion protein in which the DNA binding domain of GAL4 is fused to the protein of interest. Third: a cDNA library made in plasmids where each cDNA is fused in frame to the activation domain of GAL4.

Answer 8

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Answer 11

Ch. 17 - For each of the terms in the left column, choose...Ch. 17 - For each of the following types of gene...Ch. 17 - List five events other than transcription...Ch. 17 - Which eukaryotic RNA polymerase RNA pol I, pol II,...Ch. 17 - As shown in the following diagram, a single...Ch. 17 - You have synthesized an enhancerless GFP reporter...Ch. 17 - Prob. 7P Ch. 17 - Prob. 8P Ch. 17 - A single UAS regulates the expression of three...Ch. 17 - MyoD is a transcriptional activator that turns on...

Solution Summary: The author explains the yeast two-hybrid system method, based on activating and binding domains of the eukaryotic transcription factors.

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