Anatomy & Physiology
3rd Edition
ISBN: 9781259398629
Author: McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher: Mcgraw Hill Education,
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Textbook Question
Chapter 17, Problem 14DYB
Identify the three chemical classes of hormones, and give an example of each. Most hormones belong to which class?
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 17 Solutions
Anatomy & Physiology
Ch. 17.1 - Prob. 1LOCh. 17.1 - Prob. 1WDLCh. 17.1 - Prob. 2LOCh. 17.1 - How does the endocrine system differ from the...Ch. 17.1 - Prob. 3LOCh. 17.1 - Diabetes mellitus is noted by sustained high blood...Ch. 17.2 - Prob. 4LOCh. 17.2 - Prob. 4WDLCh. 17.2 - Prob. 5LOCh. 17.2 - Adrenocorticotropic hormone (ACTH) stimulates the...
Ch. 17.3 - Prob. 6LOCh. 17.3 - Prob. 7LOCh. 17.3 - Identify which of the following hormone categories...Ch. 17.3 - What two events or processes associated with a...Ch. 17.3 - Prob. 8LOCh. 17.3 - Prob. 9LOCh. 17.3 - Prob. 1WDTCh. 17.3 - Prob. 8WDLCh. 17.4 - Prob. 10LOCh. 17.4 - Why are carrier proteins necessary for...Ch. 17.4 - What is the added benefit of a carrier protein?Ch. 17.4 - Prob. 11LOCh. 17.4 - Prob. 12LOCh. 17.4 - Prob. 2WDTCh. 17.4 - What is the relationship of hormone synthesis to...Ch. 17.5 - Prob. 13LOCh. 17.5 - Where are lipid-soluble hormone receptors located?...Ch. 17.5 - Prob. 14LOCh. 17.5 - Prob. 13WDLCh. 17.6 - Prob. 15LOCh. 17.6 - Prob. 16LOCh. 17.6 - Prob. 3WDTCh. 17.6 - How does down-regulation of cellular receptors...Ch. 17.6 - Prob. 17LOCh. 17.6 - What effects are seen when hormones act...Ch. 17.7 - Prob. 18LOCh. 17.7 - Prob. 19LOCh. 17.7 - What is the anatomic connection between the...Ch. 17.7 - Prob. 20LOCh. 17.7 - Prob. 4WDTCh. 17.7 - Prob. 17WDLCh. 17.7 - Prob. 21LOCh. 17.7 - Prob. 22LOCh. 17.7 - Prob. 18WDLCh. 17.7 - Prob. 23LOCh. 17.7 - Prob. 24LOCh. 17.7 - Prob. 5WDTCh. 17.7 - Prob. 19WDLCh. 17.7 - Prob. 20WDLCh. 17.8 - Prob. 25LOCh. 17.8 - Prob. 26LOCh. 17.8 - Prob. 6WDTCh. 17.8 - Prob. 21WDLCh. 17.8 - Prob. 27LOCh. 17.8 - Prob. 7WDTCh. 17.8 - What is the relationship of TRH, TSH, and TH in...Ch. 17.8 - What are the primary target organs/issues of TH?...Ch. 17.8 - Prob. 28LOCh. 17.9 - Prob. 24WDLCh. 17.9 - Prob. 29LOCh. 17.9 - Prob. 30LOCh. 17.9 - Prob. 25WDLCh. 17.9 - LEARNING OBJECTIVE
31. Describe the homeostatic...Ch. 17.9 - Prob. 26WDLCh. 17.9 - What are the primary target organs/tissues of...Ch. 17.10 - Prob. 32LOCh. 17.10 - Prob. 33LOCh. 17.10 - Why is the pancreas considered both an exocrine...Ch. 17.10 - Prob. 34LOCh. 17.10 - Prob. 35LOCh. 17.10 - Prob. 8WDTCh. 17.10 - Is the stimulus for insulin and glucagon release...Ch. 17.10 - What is the stimulus, receptor, control center,...Ch. 17.10 - Which of these hormones causes release of glucose...Ch. 17.11 - LEARNING OBJECTIVE
36. Describe the general...Ch. 17.11 - How do melatonin levels change throughout the day?Ch. 17.11 - LEARNING OBJECTIVE
37. Describe the general...Ch. 17.11 - What is the primary hormone released from the...Ch. 17.11 - Prob. 38LOCh. 17.11 - Prob. 34WDLCh. 17.11 - Prob. 35WDLCh. 17.12 - Prob. 39LOCh. 17.12 - What general changes occur to the ability of...Ch. 17 - Prob. 1DYBCh. 17 - This hormones primary function is to regulate...Ch. 17 - Which of the following are components of...Ch. 17 - A hormone released from the anterior pituitary is...Ch. 17 - Prob. 5DYBCh. 17 - Prob. 6DYBCh. 17 - Glucagon has an __________ effect to insulin on...Ch. 17 - Glucocorticoids (e.g., cortisol) are produced in...Ch. 17 - Prob. 9DYBCh. 17 - All of the following hormones are released from...Ch. 17 - Prob. 11DYBCh. 17 - Prob. 12DYBCh. 17 - Explain the three mechanisms used to stimulate...Ch. 17 - Identify the three chemical classes of hormones,...Ch. 17 - Describe how local hormones differ from...Ch. 17 - Explain the function of carrier proteins in...Ch. 17 - Describe how water-soluble hormones interact with...Ch. 17 - Explain how the hypothalamus oversees and controls...Ch. 17 - Explain how the hypothalamus oversees and controls...Ch. 17 - Discuss the homeostatic system involving insulin.Ch. 17 - George is a 43-year-old construction worker who...Ch. 17 - What is the best diagnostic test to determine if...Ch. 17 - Jelena is late for work and is rushing to get out...Ch. 17 - Blood samples from a young woman named Michelle...Ch. 17 - Stephen is taking a new weight-loss supplement...Ch. 17 - Prob. 1CSLCh. 17 - Prob. 2CSLCh. 17 - Henry is a well-informed patient who is interested...
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