Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Textbook Question
Chapter 16.4, Problem 1CC
An antibiotic-resistance gene located on a bacterium’s chromosome is transferred from the bacterium to its progeny. What type of gene transfer is this?
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In a P1 transduction experiment, the P1 lysate contains phages thatcarry pieces of the host chromosomal DNA, but the lysate also containsbroken pieces of chromosomal DNA. If a P1 lysate is used to transfer chromosomal DNA to another bacterium, how could you show experimentally that the recombinant bacterium has been transduced (i.e., has taken up a P1 phage with a piece of chromosomal DNA inside) versus transformed (i.e., has taken up a piece of chromosomal DNA that is not within a P1 phage coat)?
Which of the statements is TRUE about conjugation?
a)
Plasmid DNA is transferred from a donor (F+) cell
b)
It may only be performed by competent cells
c)
DNA is always integrated into recipient chromosome
d)
It may be specialized or generalized
An F' merozygote is formed as a result of the following events:
1) the recipient cell receives genes which used to be a part of an Hfr bacterium chromosome
2) an F' bacterium excuses an F factor from another bacterium chromosome
3) an F factor that was imprecisely excised from Hfr chromosome was transferred to an F- bacterium
4)an F' bacterium conjugate with an F- bacterium
5) an F' bacterium incorporates an F factor into its chromosome
6) an F- bacterium incorporates an F factor into its chromosome
Chapter 16 Solutions
Prescott's Microbiology
Ch. 16.1 - Retrieve, Infer, Apply List three ways in which...Ch. 16.1 - Compare and contrast the means by which the...Ch. 16.1 - Give examples of intragenic and extragenic...Ch. 16.1 - Retrieve, Infer, Apply Sometimes a point mutation...Ch. 16.1 - Retrieve, Infer, Apply Why might a missense...Ch. 16.2 - How would you screen for a tryptophan auxotroph?...Ch. 16.2 - Why is a small amount of histidine added to the...Ch. 16.2 - Retrieve, Infer, Apply Describe how replica...Ch. 16.2 - Retrieve, Infer, Apply Why are mutant selection...Ch. 16.2 - Retrieve, Infer, Apply Briefly discuss how...
Ch. 16.2 - Describe how you would isolate a mutant that...Ch. 16.2 - Prob. 5CCCh. 16.3 - How is mismatch repair similar to DNA polymerase...Ch. 16.3 - How is damaged DNA recognized by the UvrAB...Ch. 16.3 - Prob. 1CCCh. 16.3 - Retrieve, Infer, Apply What role does DNA...Ch. 16.3 - Retrieve, Infer, Apply When E. coli cells are...Ch. 16.3 - Explain how the following DNA alterations and...Ch. 16.4 - An antibiotic-resistance gene located on a...Ch. 16.4 - What four fates can DNA have after entering a...Ch. 16.4 - How does homologous recombination differ from...Ch. 16.5 - What features are common to all types of...Ch. 16.5 - How does a transposon differ from an insertion...Ch. 16.5 - What is simple (cut-and-paste) transposition? What...Ch. 16.5 - What effect would you expect the existence of...Ch. 16.6 - Prob. 1MICh. 16.6 - What is bacterial conjugation and how was it...Ch. 16.6 - For F+, Hfr, and F strains of E. coli, indicate...Ch. 16.6 - Describe how F+ F and Hfr conjugation processes...Ch. 16.6 - Compare and contract F+ F and F F conjugation.Ch. 16.7 - According to this model, what would happen if DNA...Ch. 16.7 - Prob. 1CCCh. 16.7 - Describe how transformation occurs in S....Ch. 16.7 - Discuss two ways in which artificial...Ch. 16.8 - Compare the number of transducing particles that...Ch. 16.8 - Why cant the gal and bio genes be transduced by...Ch. 16.8 - Describe generalized transduction and how it...Ch. 16.8 - What is specialized transduction and how does it...Ch. 16.8 - How might one tell whether horizontal gene...Ch. 16.8 - Why doesnt a cell lyse after successful...Ch. 16.8 - Describe how conjugation, transformation, and...Ch. 16.9 - As a replicative transposon, what would happen if...Ch. 16 - Prob. 1RCCh. 16 - Prob. 2RCCh. 16 - Prob. 3RCCh. 16 - Prob. 4RCCh. 16 - Prob. 5RCCh. 16 - Prob. 6RCCh. 16 - Mutations are often considered harmful. Give an...Ch. 16 - Mistakes made during transcription affect the cell...Ch. 16 - Suppose that transduction took place when a U-tube...Ch. 16 - Suppose that you carried out a U-tube experiment...Ch. 16 - Prob. 5ALCh. 16 - Prob. 6ALCh. 16 - Prob. 7AL
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- One of the reasons why phage therapy has not been applied widely is that bacteria can become resistant to bacteriophages as well, through mutations in genes encoding for specific proteins. What would be a protein in the bacterial cell that, if mutated, would make that cell resistant to phage infection?arrow_forwardThe figure below shows a partial chromosome map of an E. coli Hfr strain. Each mark = 10 minutes between conjugation transfer time. If transfer of genes begins at “*” relative to the origin of transfer, what is one of the predicted results from this map? It would take less than 30 minutes to transfer all of the genes that are shown. gal and azi will rarely be transferred together. gal and ton will rarely be transferred together. Ten minutes after transfer of ton, lac will be transferred. This strain will produce very few gal recombinants.arrow_forwardIn which of the four processes shown can material from a bacterial genome be transferred from one cell to another? Which are forms of horizontal transmission? Place the answer(s) in the cell highlighting that process. Partial genome transfer by DNA uptake Transformation Answer Bank material from a bacterial genome horizontal gene transfer Conjugation Plasmids Conjugation Genome Plasmid transfer during conjugation Partial genome transfer during conjugation Virus Transduction Transfer as part of viral genomearrow_forward
- Which of the following statements is not applicable to conjugation? A) OF factors are transferrable plasmids passed from one cell to another during conjugation. B) O During conjugation, rolling circle replication transfers an F factor from an F+ cell to an F- cell. ) O An F factor acquired during conjugation will automatically be integrated into the recipient's chromosome. D) O Conjugation requires physical contact between cells via the formation of a sex pilus. E) O An Hfr strain is capable of transferring a portion of the chromosome during conjugation.arrow_forwardUsually, bacteria only make tryptophan when tryptophan is absent or available in low concentration. However, a particular bacterial mutation makes tryptophan all the time whether or not tryptophan is present. What could explain this phenotype? A) the terminator hairpin is unable to form B) the antiterminator hairpin is unable to form C) trpE is mutated D) trpD is mutated E) trpA is mutatedarrow_forwardMost bacterial repressor proteins are allosteric. Which of the following binds with the repressor to alter its conformation? A) promoter B) corepressor C) ATParrow_forward
- In Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets. Why was the radioactivity level of S-32 found greater outside the cells compared to the E. coli cell pellets? Explain briefly. If the experiment is repeated in the same manner but this time the phage protein coats are labelled with isotope X and the phage DNA with isotope Y, which isotope’s radioactivity will be found in greater amounts in the E. coli cell pellets after centrifugation? Explain briefly.arrow_forwardWe have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed. To experimentally determine the method of HGT that is happening, the following conditions are set up in different tubes of culture media: A) Donor and recipient strain mixed together (control - no treatment). B) Donor and recipient strains mixed together, DNase added (can digest DNA in solution, not within cells).C) Special tube containing a membrane filter (with pores that allow DNA and viruses to pass through, but not bacterial cells) that separates two compartments. Donor strain is added on one side, the recipient strain on the other (they are separated by the filter).D) Donor and recipient strains mixed together, with chemical that inactivates viruses (chemical affects bacteriophages in solution so they are unable to attach to cells). The results: Tubes A, B, and D: HGT was observed. Tube C: HGT was NOT observed. Based on this, which type of HGT was occurring? Conjugation,…arrow_forwarda bacteriophage has been isolated from the growth chambers that has evolved the capability to the methylate its own DNA when it is replicated inside the host bacterium. why is this a problem?arrow_forward
- A plasmid may A) replicate independently of the chromosome. B) be transferred cell-to-cell during conjugation. C) be integrated into the chromosome. D) replicate independently of the chromosome, integrate into the chromosome, or be transferred cell-to-cell during conjugation.arrow_forwardWhich of the following statement about conjugation is TRUE: During F+ to F- mating, the donor becomes F-. During the Hfr to F- transfer, the recipient becomes F+. The whole bacteria genome may get transferred during Hfr mating. During the Hfr to F- transfer, some recipients become recombinants in which transferred fragment recombines with homologous region in the genome.arrow_forwardSome mutations that occur in bacteria can cause the loss of phage receptors, and these bacteria become phage resistant. In order for a phage to infect the host bacterium, it is preferred that the cell wall is newly synthesized.why ?arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license