Microbiology Fundamentals: A Clinical Approach - Standalone book
Microbiology Fundamentals: A Clinical Approach - Standalone book
2nd Edition
ISBN: 9780078021046
Author: Marjorie Kelly Cowan Professor, Jennifer Bunn
Publisher: McGraw-Hill Education
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Chapter 15, Problem 8MCQ
Summary Introduction

Introduction:

Western Blotting is a technique used for identification of foreign antigens by running the samples of unknown proteins on denaturing SDS-polyacrylamide gel. These samples are then transferred to a nitrocellulose membrane or PVDF membrane. Staining is done with primary antibodies, which bind to specific proteins. Primary antibodies then bind to the secondary antibodies, which are conjugated to horseradish peroxidase. It is used for analyzing the bands on an X-ray film.

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