Human Anatomy (8th Edition)
8th Edition
ISBN: 9780134243818
Author: Elaine N. Marieb, Patricia Brady Wilhelm, Jon B. Mallatt
Publisher: PEARSON
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Chapter 15, Problem 7CRCAQ
Summary Introduction
To review:
The effects of destruction of ciliary, pterygopalatine, and submandibular ganglia.
Introduction:
Ganglion is an ovoid structure that is composed of cell bodies of the postganglionic neuron. It is a center for interaction between motor neurons of visceral/autonomic nervous system. The sympathetic and parasympathetic ganglia are present in the autonomic nervous system and these ganglia consist of cell bodies belonging to the sympathetic and parasympathetic neurons.
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a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Chapter 15 Solutions
Human Anatomy (8th Edition)
Ch. 15 - Which fibers in the motor division of the PNS are...Ch. 15 - Prob. 2CYUCh. 15 - Where are the sympathetic ganglia located? Where...Ch. 15 - Are visceral sensory fibers considered part of the...Ch. 15 - Which spinal nerves carry parasympathetic...Ch. 15 - Prob. 6CYUCh. 15 - Prob. 7CYUCh. 15 - Why are white rami communicantes located only on...Ch. 15 - What is the general effect of sympathetic...Ch. 15 - Which types of autonomic fibers (preganglionic,...
Ch. 15 - Prob. 11CYUCh. 15 - Prob. 12CYUCh. 15 - Which region of the CNS is the main control center...Ch. 15 - Which embryonic tissue forms the postganglionic...Ch. 15 - Which division of the ANS is deficient in...Ch. 15 - Which of the following does not characterize the...Ch. 15 - For each of the following terms or phrases, write...Ch. 15 - The thoracic splanchnic nerves contain which kind...Ch. 15 - The collateral ganglia contain which kind of cell...Ch. 15 - Preganglionic sympathetic neurons develop from (a)...Ch. 15 - Prob. 6RQCh. 15 - The white rami communicantes contain what kind of...Ch. 15 - Collateral sympathetic ganglia are involved. with...Ch. 15 - Is the visceral sensory nervous system part of the...Ch. 15 - (a) Describe the anatomical relationship of the...Ch. 15 - What effect does sympathetic activation have on...Ch. 15 - Which of the effects listed in question 11 would...Ch. 15 - A friend asks you how the parasympathetic...Ch. 15 - What manifestations of decreased ANS efficiency...Ch. 15 - The students in anatomy class were having...Ch. 15 - Describe the sympathetic pathways to the (a)...Ch. 15 - Prob. 17RQCh. 15 - Prob. 18RQCh. 15 - Prob. 19RQCh. 15 - How do the somatic motor and visceral motor (ANS)...Ch. 15 - A 1-year-old infant has a swollen abdomen and is...Ch. 15 - Prob. 2CRCAQCh. 15 - Prob. 3CRCAQCh. 15 - Prob. 4CRCAQCh. 15 - Prob. 5CRCAQCh. 15 - Prob. 6CRCAQCh. 15 - Prob. 7CRCAQCh. 15 - Prob. 8CRCAQ
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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