BIOL 107 LAB PKG W/LAB SG +ACCESS >IC<
12th Edition
ISBN: 9781259827082
Author: Mader
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 15, Problem 6A
Organisms
a. compete with other members of their species.
b. differ in fitness.
c. are adapted to their environment.
d. are related by descent from common ancestors.
e. All of these are correct.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 15 Solutions
BIOL 107 LAB PKG W/LAB SG +ACCESS >IC<
Ch. 15.1 - Define catastrophism and identify who proposed...Ch. 15.1 - Evaluate Lamarck’s idea of “inheritance of...Ch. 15.1 - Construct a timeline of the history of...Ch. 15.2 - List the three categories of observations made by...Ch. 15.2 - Summarize the components of Darwin’s theory of...Ch. 15.2 - Prob. 3CYPCh. 15.3 - Prob. 1CYPCh. 15.3 - Summarize the difference between homologous and...Ch. 15.3 - Explain how biomolecules support the theory of...Ch. 15.3 - Prob. 4CYP
Ch. 15 - Prob. 1NS.1QCCh. 15 - How does the tree of life support Darwin’s theory...Ch. 15 - Which of these pairs is mismatched? a. Charles...Ch. 15 - Prob. 2ACh. 15 - Prob. 3ACh. 15 - Why was it helpful to Darwin to learn that Lyell...Ch. 15 - Prob. 5ACh. 15 - Organisms a. compete with other members of their...Ch. 15 - Prob. 7ACh. 15 - A key biogeographical observation made by Darwin...Ch. 15 - Prob. 9ACh. 15 - Humans are able to cause organisms to evolve...Ch. 15 - Prob. 11ACh. 15 - Prob. 12ACh. 15 - Prob. 13ACh. 15 - All vertebrate embryos have pharyngeal pouches....Ch. 15 - Transitional fossils have been found between some...Ch. 15 - Mutations occur at random and can increase within...Ch. 15 - 2. A cotton farmer applies a new insecticide...Ch. 15 - Prob. 3TSCh. 15 - Both Darwin and Wallace, while observing life on...
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