Interpretation:
The reason for the low mobility of chlorophylls on column chromatography is to be determined.
Concept introduction:
Column chromatography is a technique in
Answer to Problem 1Q
Chlorophylls are polar compounds. Therefore, chlorophylls tend to attach with the silica in the column which are also polar. Carotene are less polar compounds which easily elute through the column.
Explanation of Solution
In column chromatography, a long column is used that is packed with a polar compound such as silica or alumina. This compound acts as the stationary phase of the process.Silica (or alumina) is a polar compound. Usually, polar compound interact with other polar compounds due to attractions between them. When sample is passed which contains both silica and alumina through this column, chlorophyll tends to retain in the column while carotene elutes first. This is mainly because chlorophyll is a polar compound that retains in the column. Carotene is a less polar compound that elutes from the column.
The
Chlorophyll is a polar compound while carotene is a less polar compound. In the presence of a polar stationary phase, chlorophyll moves slower than carotene because chlorophyll attracts to the polar stationary phase.
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Chapter 15 Solutions
EBK A SMALL SCALE APPROACH TO ORGANIC L
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