BROOKER BIO 3-HOLE PUNCH W/CONNECT BUND
5th Edition
ISBN: 9781264065615
Author: BROOKER
Publisher: MCGRAW-HILL LEARNING SOLN.(CC)
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Textbook Question
Chapter 1.5, Problem 1CC
What is the purpose of a control group in hypothesis testing?
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The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
There is currently a H5N1 cattle outbreak in North America.
According to the CDC on Feb 26*: "A multistate outbreak of HPAI A(H5N1) bird flu in dairy cows was first reported on March 25, 2024. This is the first time that these bird flu viruses had been found in cows. In the United States, since 2022, USDA has reported HPAI A(H5N1) virus detections in more than 200 mammals."
List and describe two mechanisms that could lead to this H5N1 influenza strain evolving to spread in human:
Mechanisms 1:
Mechanisms 2:
For the mutations to results in a human epidered they would need to change how the virus interacts with the human host. In the case of mutations that may promote an epidemic, provide an example for:
a protein that might incur a mutation:
how the mutation would change interactions with cells in the respiratory tract (name the receptor on human cells)
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You have a bacterial strain with the CMU operon:
a) As shown in the image below, the cmu operon encodes a peptide (Pep1), as well as a
kinase and regulator corresponding to a two-component system. The cmu operon is
activated when Pep 1 is added to the growth media.
Pep1 is a peptide that when added extracellularly leads to activation of the Cmu operon.
Pep1 cmu-kinase cmu-regulator
You also have these genetic components in other strains:
b) An alternative sigma factor, with a promoter activated by the cmu-regulator, that control a
series of multiple operons that together encode a transformasome (cellular machinery
for transformation).
c) the gene cl (a repressor).
d) the promoter X, which includes a cl binding site (and in the absence of cl is active).
e) the gene gp (encoding a green fluorescence protein).
Using the cmu operon as a starting point, and assuming you can perform cloning to rearrange
any of these genomic features, how would you use one or more of these to modify the…
Chapter 1 Solutions
BROOKER BIO 3-HOLE PUNCH W/CONNECT BUND
Ch. 1.1 - At which level of biological organization would...Ch. 1.3 - What is the relationship between biological...Ch. 1.3 - How does the concept of a tree of life differ from...Ch. 1.3 - Biologists sometimes say that the genome is the...Ch. 1.4 - Figure 1.10 The three domains of life. Two of...Ch. 1.4 - Why is it useful to place organisms into taxonomic...Ch. 1.5 - What is the purpose of a control group in...Ch. 1.5 - Explain how discovery-based science helped...Ch. 1.6 - Discuss the difference between discovery-based...Ch. 1.6 - What are the steps in the scientific method, also...
Ch. 1.6 - Prob. 3EQCh. 1.6 - Prob. 1CSCh. 1.6 - Core Skill: Modeling This modeling challenge asks...Ch. 1 - Which of the following is not a core concept of...Ch. 1 - Populations of organisms change over the course of...Ch. 1 - Prob. 3TYCh. 1 - Which of the following is an example of horizontal...Ch. 1 - The scientific name for humans is Homo sapiens....Ch. 1 - The complete genetic makeup of an organism a....Ch. 1 - Prob. 7TYCh. 1 - Prob. 8TYCh. 1 - Prob. 9TYCh. 1 - What is the purpose of using a control group in a...Ch. 1 - Of the five core concepts of biology described in...Ch. 1 - Explain how it is possible for evolution to result...Ch. 1 - Prob. 3CQCh. 1 - Discuss whether or not you think that theories in...Ch. 1 - Prob. 2COQ
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- You have identified a new species of a Gram-positive bacteria. You would like to screen their genome for all proteins that are covalently linked to the cell wall. You have annotated the genome, so that you identified all the promoters, operons, and genes sequences within the operons. Using these features, what would you screen for to identify a set of candidates for proteins covalently linked to the bacterial cell wall.arrow_forwardBelow is a diagram from a genomic locus of a bacterial genome. Each arrow represents a coding region, and the arrowheads indicate its orientation in the genome. The numbers are randomly assigned. Draw the following features on the diagram, and explain your rationale for each feature: 10 12 合會會會會長 6 a) Expected transcriptions, based on known properties of bacterial genes and operons. How many proteins are encoded in each of the transcripts? b) Location of promoters (include rationale) c) Location of transcriptional terminators (include rationale) d) Locations of Shine-Dalgarno sequences (include rationale)arrow_forwardSample excuse letter in school class for the reasons of headaches and dysmenorrhea caused by menstrual cyclearrow_forward
- How do the muscles on the foot work to balance on an ice skate, specifically the triangle of balance and how does it change when balancing on an ice skate? (Refer to anatomy, be specific)arrow_forwardWhich of the following is NOT an example of passive immunization? A. Administration of tetanus toxoid B. Administration of hepatitis B immunoglobulin C. Administration of rabies immunoglobulin D. Transfer of antibodies via plasma therapyarrow_forwardTranscription and Translation 1. What is the main function of transcription and translation? (2 marks) 2. How is transcription different in eukaryotic and prokaryotic cells? (2 marks) 3. Explain the difference between pre-mRNA and post-transcript mRNA. (2 marks) 4. What is the function of the following: (4 marks) i. the cap ii. spliceosome iii. Poly A tail iv. termination sequence 5. What are advantages to the wobble feature of the genetic code? (2 marks) 6. Explain the difference between the: (3 marks) i. A site & P site ii. codon & anticodon iii. gene expression and gene regulation 7. Explain how the stop codon allows for termination. (1 mark) 8. In your own words, summarize the process of translation. (2 marks)arrow_forward
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