Chapter 15, Problem 12TYU

Summary Introduction

To determine: The problems that might arise while producing eukaryotic protein in a bacterium and how the usage of transgenic plants or animals may help to solve these problems.

Concept introduction: Recombinant DNA technology allows addition of a eukaryotic gene into a bacterial plasmid vector. This recombinant plasmid expresses the eukaryotic gene by synthesizing a large quantity of protein encoded by it. There are certain hurdles in this, as the gene expression in eukaryotes and prokaryotes is different.