Biology (MindTap Course List)
10th Edition
ISBN: 9781285423586
Author: Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher: Cengage Learning
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Chapter 15, Problem 12TYU
Summary Introduction
To determine: The problems that might arise while producing eukaryotic protein in a bacterium and how the usage of transgenic plants or animals may help to solve these problems.
Concept introduction: Recombinant DNA technology allows addition of a eukaryotic gene into a bacterial plasmid vector. This recombinant plasmid expresses the eukaryotic gene by synthesizing a large quantity of protein encoded by it. There are certain hurdles in this, as the gene expression in eukaryotes and prokaryotes is different.
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Quiz: References, Quotations, and Formatting
If you transplant trunk neural crest into the cranial neural crest region of a developing embryo, will you see the donor tissue form cartilage?
Does the neural crest only give rise to two cells in the developing embryo, and is essential for lamprey to develop their jaw structure?
Does a multipotent neural crest cell that is receiving Wnt signals become a Chromaffin cell?
Chapter 15 Solutions
Biology (MindTap Course List)
Ch. 15.1 - Prob. 1LOCh. 15.1 - Prob. 1CCh. 15.1 - Prob. 2LOCh. 15.1 - Prob. 3LOCh. 15.1 - Prob. 4LOCh. 15.1 - What do genomic DNA libraries and cDNA libraries...Ch. 15.1 - Prob. 3CCh. 15.2 - Prob. 5LOCh. 15.2 - Prob. 6LOCh. 15.2 - Prob. 7LO
Ch. 15.2 - Prob. 1CCh. 15.2 - Prob. 2CCh. 15.3 - Describe how genome-wide association studies have...Ch. 15.3 - Explain how targeted gene silencing and knockout...Ch. 15.3 - Prob. 1CCh. 15.4 - Describe at least one important application of DNA...Ch. 15.4 - Prob. 1CCh. 15.4 - What are short tandem repeats (STRs), and why are...Ch. 15.4 - Why do gene targeting and mutagenesis screening in...Ch. 15.5 - Describe at least two safety issue associated with...Ch. 15.5 - What are some of the environment concerns...Ch. 15 - A plasmid (a) can be used as a DNA vector (b) is a...Ch. 15 - DNA molecules with complementary sticky ends...Ch. 15 - Prob. 3TYUCh. 15 - Which technique rapidly replicated specific DNA...Ch. 15 - Prob. 5TYUCh. 15 - A cDNA clone contains (a) introns (b) exons (c)...Ch. 15 - Prob. 7TYUCh. 15 - Gel electrophoresis separates nucleic acids on the...Ch. 15 - Prob. 9TYUCh. 15 - DNA molecular with complementary sticky ends...Ch. 15 - These highly polymorphic molecular markers are...Ch. 15 - Prob. 12TYUCh. 15 - Prob. 13TYUCh. 15 - Prob. 14TYUCh. 15 - EVOLUTION LINK DNA technology, such as the...Ch. 15 - SCIENCE, TECHNOLOGY, AND SOCIETY What are some...
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- Using quail and chick embryos, quail-specific antibody and fluorescent tissue-specific antibodies, design an experiment where you investigate the tissues the cranial neural crest can give rise to. What are four derivatives of the cranial neural crest that you expect to see in the resulting chimeric embryos?arrow_forwardDoes the neural crest have to undergo epithelial to mesenchymal transition prior to migration through the developing embryo? Does the neural crest differentiate into different cell types based on their axial position along the anterior and posterior axis?arrow_forwardUsing quail and chicken embryos, what kind of experiment would you conduct to test if rib forming somites have their axial identity specified before segmentation? How do we know this phenotype is due to axial identity being specified before segmentation and not due to our experimental method?arrow_forward
- 8. Aerobic respiration of a 5 mM solution of tripeptide that is composed of the following three amino acids; alanine, leucine and isoleucine. Alanine breaks down to pyruvate, leucine breaks down to Acetyl-CoA and isoleucine breaks down to succinyl-CoA. Alanine NADH FADH2 OP ATP SLP ATP Total ATP Leucine Isoleucine Totals Show your work using dimensional analysis here: 4arrow_forward9. Aerobic respiration of one lipid molecule. The lipid is composed of one glycerol molecule connected to two fatty acid tails. One fatty acid is 12 carbons long and the other fatty acid is 18 carbons long in the figure below. Use the information below to determine how much ATP will be produced from the glycerol part of the lipid. Then, in part B, determine how much ATP is produced from the 2 fatty acids of the lipid. Finally put the NADH and ATP yields together from the glycerol and fatty acids (part A and B) to determine your total number of ATP produced per lipid. Assume no other carbon source is available. fatty acids glycerol 18 carbons 12 carbons 0=arrow_forwardinfluences of environment on the phenotype.arrow_forward
- What is the difference between codominance and phenotypic plasticity?arrow_forwardExplain the differences between polygeny and pleiotropy,arrow_forwardIf using animals in medical experiments could save human lives, is it ethical to do so? In your answer, apply at least one ethical theory in support of your position.arrow_forward
- You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?arrow_forwardYou decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.arrow_forwardYou implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb? Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license