Biochemistry
Biochemistry
6th Edition
ISBN: 9781337359573
Author: Reginald H. Garrett; Charles M. Grisham
Publisher: Cengage Learning US
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Chapter 14, Problem 9P

Answers to all problems are at the end οf this book. Detailed solutions are available in the Student Solutions Manual. Study Guide, and Problems Book.

Understanding the Implications of Transition State Stabilization

As noted in Section 14.4. a true transition state can bind to an enzyme active site with a KT as Low as 7 X 10-26 M. This is a remarkable number, with interesting consequences. Consider a hypothetical solution of an enzyme in equilibrium with a ligand that binds with a KD of 10-27 M. If the concentration of free enzyme. [EL]. is equal to the concentration of the enzyme-ligand complex. [EL], what would [L]. the concentration of free ligand, be? Calculate the volume of solution that would hold one molecule of free ligand at this concentration.

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