Chapter 14, Problem 8A

When mutations that affected DNA replication were isolated, two kinds were found. In cultures that were not synchronized (that is, not all dividing at the same time), one class put an immediate halt to replication, whereas the other put a much slower stop to the process. The first class affects functions at the replication fork like polymerase and primase. The second class affects functions necessary for

a. elongation on the lagging but not the leading strand.

b. elongation on the leading but not the lagging strand.

c. initiation: cells complete replication but cannot start a new round.

d. the sliding clamp: loss makes the polymerase slower.