The transformation of succinate into glucose and its stoichiometry. Introduction: A toxic glycoside, phloridzin obtained from the bark of pear tree blocks the glucose absorption form kidney tubules and cause the blood glucose to be excreted in urine.

Question

Want to see more full solutions like this?

Answer 1

Question

Chapter 14, Problem 28P

Summary Introduction

To determine: The transformation of succinate into glucose and its stoichiometry.

Introduction:

A toxic glycoside, phloridzin obtained from the bark of pear tree blocks the glucose absorption form kidney tubules and cause the blood glucose to be excreted in urine.

Expert Solution & Answer

Explanation of Solution

Explanation:

Phloridzin blocks the reabsorption of glucose in the tubules of kidney which allows excretion of glucose in urine. The ingested succinate can be converted into glucose through the process of gluconeogenesis.

Succinate is first converted into oxaloacetate through series of reactions. Then the oxaloacetate is transported into cytosol for its conversion into phosphoenolpyruvate by enzyme, PEP carboxykinase. In the cytosol, PEP is converted into glucose through gluconeogenesis.

The mechanism (stoichiometry) of the reaction is as follows:

Succinate→Fumerate→Malate→Oxaloacetate→PEP carboxykinasePhosphoenolpyruvate ↓ ↓ Glucose

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

Draw a diagram to demonstrate 3' to 5' exonuclease activity?

1. Write the piece of mRNA that would code for the amino acid sequence NH3 - met - tyr - cys- his- CO2. Label the 5' and 3' ends. (diagram attached) 2.

You were tasked with purifying an untagged transcription factor (molecular weight 65,000Da, isoelectric point unknown) from a contaminant Protein A (molecular weight 50,000 Da, isoelectric point 5.0). You were also instructed to use the protein for crystallization studies after purification.i) In the initial purification step, which type of chromatography should you attempt? Explain your choice and specify the requirements of the buffer solution you should use. ii) Analyzing the proteins recovered from step i) using SDS-PAGE, you still observed a faint band of Protein A in addition to the transcription factor band. Given the limited time available for further purification, you must choose ONE appropriate chromatography method to maximize the chances of separating the two proteins as well as your crystallization studies. Provide a detailed explanation of your selection and the techniques/strategies you would employ to achieve this.

Answer 2

Question

Chapter 14, Problem 28P

Summary Introduction

To determine: The transformation of succinate into glucose and its stoichiometry.

Introduction:

A toxic glycoside, phloridzin obtained from the bark of pear tree blocks the glucose absorption form kidney tubules and cause the blood glucose to be excreted in urine.

Expert Solution & Answer

Explanation of Solution

Explanation:

Phloridzin blocks the reabsorption of glucose in the tubules of kidney which allows excretion of glucose in urine. The ingested succinate can be converted into glucose through the process of gluconeogenesis.

Succinate is first converted into oxaloacetate through series of reactions. Then the oxaloacetate is transported into cytosol for its conversion into phosphoenolpyruvate by enzyme, PEP carboxykinase. In the cytosol, PEP is converted into glucose through gluconeogenesis.

The mechanism (stoichiometry) of the reaction is as follows:

Succinate→Fumerate→Malate→Oxaloacetate→PEP carboxykinasePhosphoenolpyruvate ↓ ↓ Glucose

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Question

Chapter 14, Problem 28P

Summary Introduction

To determine: The transformation of succinate into glucose and its stoichiometry.

Introduction:

A toxic glycoside, phloridzin obtained from the bark of pear tree blocks the glucose absorption form kidney tubules and cause the blood glucose to be excreted in urine.

Expert Solution & Answer

Explanation of Solution

Explanation:

Phloridzin blocks the reabsorption of glucose in the tubules of kidney which allows excretion of glucose in urine. The ingested succinate can be converted into glucose through the process of gluconeogenesis.

Succinate is first converted into oxaloacetate through series of reactions. Then the oxaloacetate is transported into cytosol for its conversion into phosphoenolpyruvate by enzyme, PEP carboxykinase. In the cytosol, PEP is converted into glucose through gluconeogenesis.

The mechanism (stoichiometry) of the reaction is as follows:

Succinate→Fumerate→Malate→Oxaloacetate→PEP carboxykinasePhosphoenolpyruvate ↓ ↓ Glucose

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Question

Chapter 14, Problem 28P

Summary Introduction

To determine: The transformation of succinate into glucose and its stoichiometry.

Introduction:

A toxic glycoside, phloridzin obtained from the bark of pear tree blocks the glucose absorption form kidney tubules and cause the blood glucose to be excreted in urine.

Expert Solution & Answer

Explanation of Solution

Explanation:

Phloridzin blocks the reabsorption of glucose in the tubules of kidney which allows excretion of glucose in urine. The ingested succinate can be converted into glucose through the process of gluconeogenesis.

Succinate is first converted into oxaloacetate through series of reactions. Then the oxaloacetate is transported into cytosol for its conversion into phosphoenolpyruvate by enzyme, PEP carboxykinase. In the cytosol, PEP is converted into glucose through gluconeogenesis.

The mechanism (stoichiometry) of the reaction is as follows:

Succinate→Fumerate→Malate→Oxaloacetate→PEP carboxykinasePhosphoenolpyruvate ↓ ↓ Glucose

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Chapter 14, Problem 28P

Summary Introduction

To determine: The transformation of succinate into glucose and its stoichiometry.

Introduction:

A toxic glycoside, phloridzin obtained from the bark of pear tree blocks the glucose absorption form kidney tubules and cause the blood glucose to be excreted in urine.

Expert Solution & Answer

Explanation of Solution

Explanation:

Phloridzin blocks the reabsorption of glucose in the tubules of kidney which allows excretion of glucose in urine. The ingested succinate can be converted into glucose through the process of gluconeogenesis.

Succinate is first converted into oxaloacetate through series of reactions. Then the oxaloacetate is transported into cytosol for its conversion into phosphoenolpyruvate by enzyme, PEP carboxykinase. In the cytosol, PEP is converted into glucose through gluconeogenesis.

The mechanism (stoichiometry) of the reaction is as follows:

Succinate→Fumerate→Malate→Oxaloacetate→PEP carboxykinasePhosphoenolpyruvate ↓ ↓ Glucose

Answer 6

Chapter 14, Problem 28P

Summary Introduction

To determine: The transformation of succinate into glucose and its stoichiometry.

Introduction:

A toxic glycoside, phloridzin obtained from the bark of pear tree blocks the glucose absorption form kidney tubules and cause the blood glucose to be excreted in urine.

Expert Solution & Answer

Explanation of Solution

Explanation:

Phloridzin blocks the reabsorption of glucose in the tubules of kidney which allows excretion of glucose in urine. The ingested succinate can be converted into glucose through the process of gluconeogenesis.

Succinate is first converted into oxaloacetate through series of reactions. Then the oxaloacetate is transported into cytosol for its conversion into phosphoenolpyruvate by enzyme, PEP carboxykinase. In the cytosol, PEP is converted into glucose through gluconeogenesis.

The mechanism (stoichiometry) of the reaction is as follows:

Succinate→Fumerate→Malate→Oxaloacetate→PEP carboxykinasePhosphoenolpyruvate ↓ ↓ Glucose

Answer 7

Chapter 14, Problem 28P

Summary Introduction

To determine: The transformation of succinate into glucose and its stoichiometry.

Introduction:

A toxic glycoside, phloridzin obtained from the bark of pear tree blocks the glucose absorption form kidney tubules and cause the blood glucose to be excreted in urine.

Answer 8

Expert Solution & Answer

Explanation of Solution

Explanation:

Phloridzin blocks the reabsorption of glucose in the tubules of kidney which allows excretion of glucose in urine. The ingested succinate can be converted into glucose through the process of gluconeogenesis.

Succinate is first converted into oxaloacetate through series of reactions. Then the oxaloacetate is transported into cytosol for its conversion into phosphoenolpyruvate by enzyme, PEP carboxykinase. In the cytosol, PEP is converted into glucose through gluconeogenesis.

The mechanism (stoichiometry) of the reaction is as follows:

Succinate→Fumerate→Malate→Oxaloacetate→PEP carboxykinasePhosphoenolpyruvate ↓ ↓ Glucose

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Ch. 14 - Prob. 1P Ch. 14 - Prob. 2P Ch. 14 - Prob. 3P Ch. 14 - Prob. 4P Ch. 14 - Prob. 5P Ch. 14 - Prob. 6P Ch. 14 - Prob. 7P Ch. 14 - Prob. 8P Ch. 14 - Prob. 9P Ch. 14 - Prob. 10P

The transformation of succinate into glucose and its stoichiometry. Introduction: A toxic glycoside, phloridzin obtained from the bark of pear tree blocks the glucose absorption form kidney tubules and cause the blood glucose to be excreted in urine.

Concept explainers

Videos

To determine: The transformation of succinate into glucose and its stoichiometry.

Introduction:

A toxic glycoside, phloridzin obtained from the bark of pear tree blocks the glucose absorption form kidney tubules and cause the blood glucose to be excreted in urine.

Explanation of Solution

Want to see more full solutions like this?

Chapter 14 Solutions

The transformation of succinate into glucose and its stoichiometry. Introduction: A toxic glycoside, phloridzin obtained from the bark of pear tree blocks the glucose absorption form kidney tubules and cause the blood glucose to be excreted in urine.

Concept explainers

Videos

To determine: The transformation of succinate into glucose and its stoichiometry. Introduction: A toxic glycoside, phloridzin obtained from the bark of pear tree blocks the glucose absorption form kidney tubules and cause the blood glucose to be excreted in urine.

Explanation of Solution

Want to see more full solutions like this?

Chapter 14 Solutions

To determine: The transformation of succinate into glucose and its stoichiometry.

Introduction:

A toxic glycoside, phloridzin obtained from the bark of pear tree blocks the glucose absorption form kidney tubules and cause the blood glucose to be excreted in urine.