Biology: Life on Earth
10th Edition
ISBN: 9780321729712
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: Benjamin Cummings
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Textbook Question
Chapter 13, Problem 3RQ
What is a restriction enzyme? How can restriction enzymes be used to splice a piece of human DNA into a plasmid?
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What is a restriction enzyme? How can restriction enzymes be used to splice a piece of human DNA into a plasmid?
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Chapter 13 Solutions
Biology: Life on Earth
Ch. 13 - Are organisms that contain DNA that has been...Ch. 13 - 2._________ is the process whereby bacteria pick...Ch. 13 - 3. The______ is a technique for multiplying DNA in...Ch. 13 - Matching DNA samples in forensics uses a specific...Ch. 13 - Prob. 5FTBCh. 13 - Prob. 1RQCh. 13 - 2. What is a plasmid? How are plasmids involved in...Ch. 13 - 3. What is a restriction enzyme? How can...Ch. 13 - 4. Describe the polymerase chain reaction.
Ch. 13 - What is a short tandem repeat? How are short...
Ch. 13 - How does gel electrophoresis separate pieces of...Ch. 13 - How are DNA probes used to identify specific...Ch. 13 - Prob. 8RQCh. 13 - Describe several uses of genetic engineering in...Ch. 13 - Prob. 10RQCh. 13 - BioEthics In a 2004 Web survey conducted by the...Ch. 13 - 1. As you may know, many insects have evolved...Ch. 13 - BioEthics Discuss the ethical issues that surround...Ch. 13 - 2. All children born with X-linked SCID are boys....
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- What is the job of the restriction enzyme?arrow_forwardWhat are palindromes, and how are they related to restriction endonucleases? Why are they useful for inserting one piece of DNA into another?arrow_forwardIn the formation of recombinant DNA, a restriction endonuclease cuts a bacterial plasmid to give sticky ends. The DNA segments that are to be added to the plasmid are cleaved with the same restriction endonuclease. What aresticky ends and why is it important that the target DNA and the plasmid it will be incorporated into have complementary sticky ends?arrow_forward
- Besides their role in genetic engineering, what else do plasmids can plasmids be used for? How are restriction enzymes used in genetic engineering?arrow_forwardDescribe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description.arrow_forwardYou have a recombinant plasmid containing a vector and a segment of foreign DNA, both equal sizes. Draw a picture of this recombinant plasmid labeling foreign and vector regions. Where the foreign DNA meets the vector, there is a cut site for restriction enzyme ABC1. When the recombinant plasmid is cut by ABC1, how many fragments do you expect to be produced? Identify these fragments.arrow_forward
- Restriction sites are palindromic; that is, they read the same in the5' to 3' direction on each strand of DNA. What is the advantage ofhaving restriction sites organized this way?arrow_forwardWhat are the three types of DNA ends that can be generated after cutting DNA with restriction enzymes? What reaction is catalyzed by DNA ligase?arrow_forwardIn making recombinant DNA, what is the benefit of using a restriction enzyme that cuts DNA in a staggered fashion?arrow_forward
- DEFINE THE FOLLOWING: 1) restriction enzyme 2) plasmid 3) recombinant DNAarrow_forwardWhich of the following is necessary for a PCR reaction to proceed? a) the sequence of the ends of the DNA to be amplified must be known. b) the sequence of restriction endonuclease recognition sites in the DNA to be amplified and in the plasmid, where the amplified DNA fragment will be cloned must be known. c) The complete sequence of the DNA to be amplified must be known. d) The sequence of restriction endonuclease recognition sites in the DNA to be amplified must be knownarrow_forwardThis plasmid was digested using different restriction enzymes whose sites have been mapped. The plasmid is 7896 base pairs long. This is a long question so u can count this as two or even three but please answer the question? Determine the size (base pairs) and number of fragments that would be produced if the plasmid was digested with the following enzymes: a) EcoRI b) BamHI c) HindIII d) EcoRI and HindIII e)EcoRI, HindIII, and BamHI *Hint- this is actually an EASY question, since the restriction map is already drawn for you!arrow_forward
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