Chapter 13, Problem 3MIR

Molecular Biology Question You are working to characterize a novel protein in mice. Analysis shows that high levels of the primary transcript that codes for this protein are found in tissue from the brain, muscle, liver, and pancreas. However, an antibody that recognizes the C-terminal portion of the protein indicates that the protein is present in brain, muscle, and liver, but not in the pancreas. What is the most likely explanation for this result?

Molecular Biology Explain/discuss how “slow stop” and “quick/fast stop” mutants wereused to identify different protein involved in DNA replication in E. coli.

Molecular Biology Question A gene that codes for a protein was removed from a eukaryotic cell and inserted into a prokaryotic cell. Although the gene was successfully transcribed and translated, it produced a different protein than it produced in the eukaryotic cell. What is the most likely explanation?

Molecular Biology LIST three characteristics of origins of replication

Molecular Biology Question Please help. Thank you For E coli DNA polymerase III, give the structure and function of the b-clamp sub-complex. Describe how the structure of this sub-complex is important for it’s function.

Molecular Biology LIST three characteristics of DNA Polymerases

Molecular Biology RNA polymerase core enzyme structure contains what subunits? To form holo enzyme, sigma factor is added to core. What is the name of the structure formed? Give the detailed structure of sigma factor and the function of eachdomain. Please help. Thank you

Molecular Biology You have a single bacterial cell whose DNA is labelled with radioactiveC14. After 5 rounds of cell division, how may cells will contain radioactive DNA? Please help. Thank you

1. Explain the structure and properties of atoms and chemical bonds (especially how they relate to DNA and proteins). Also add some pictures.